摘要
目的：观察应用β淀粉样蛋白（ Aβ）后大鼠海马诱导型一氧化氮合酶（ iNOS）的表达和突触超微结构的变化及雷公藤内酯醇对其的影响。方法21只SD雄性大鼠等分成对照组、模型组、用药组。在大鼠左侧海马定向注射凝聚态 Aβ1～40作为模型组，注射等量生理盐水设为对照组，大鼠在海马注射凝聚态Aβ1～40后腹腔注射雷公藤内酯醇为用药组。应用免疫组织化学方法检测各组大鼠海马 iNOS的表达；应用透射电镜观察各组大鼠海马突触超微结构的变化。结果免疫组织化学染色显示，模型组大鼠海马iNOS阳性细胞数和平均光密度明显高于对照组（P＜0．01），用药组大鼠海马iNOS阳性细胞平均光密度较模型组明显降低（P＜0．05）。透射电镜结果显示，模型组大鼠海马神经毡内突触和突触小泡数量较对照组减少，突触后致密物厚度变薄；用药组大鼠海马神经毡内突触和突触小泡数量较模型组增多，突触后致密物增厚。结论雷公藤内酯醇可以抑制Aβ诱导的大鼠海马iNOS的表达，对突触超微结构的损伤有一定的改善作用。
Objective To explore the effects of triptolide on the expression of inducible nitric oxide synthase （ iNOS） and synaptic ultrastructure in hippocampus of model rats with Alzheimer＇s disease （AD）.Methods Twenty-one male SD rats were equally divided into control ,AD model and triptolide-treated groups .The AD model group were made with unilateral microinjection of aggregated beta-amyloid pro-tein（ Aβ） 1～40 into hippocampus in rats and the control group rats were injected with normal saline in the same way .The triptolide-treated group rats were administered triptolide intraperitoneally after microinjection of aggregated Aβ1～40 into hippocampus .The iNOS expression and synaptic ultrastructure in hippocampus of each group were assayed by immunohistochemical staining and transmission electron microscope . Results Immunohistochemically ,the cell number and average optical density of iNOS positive staining in the AD model group were higher than those of control group （P〈0.01）,the average optical density of iNOS positive staining in the triptolide-treated group were lower than that of AD model group（P〈0.05）.The number of synapses and synaptic vesicles and the thickness of postsynaptic density of hippocampal neuro -pil in the AD model group were lower than those of control group .The number of synapses and synaptic vesicles and the thickness of postsyn -aptic density of hippocampal neuropil in the triptolide-treated group were higher than those of AD model group .Conclusions Triptolide could inhibit iNOS expression and alleviate the degeneration of synapses in hippocampus of model rats with AD .
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2014年第22期6379-6381,共3页
Chinese Journal of Gerontology
基金
国家自然科学基金(30660073)
江西省教育厅科研基金(赣教技字〔2007〕87号)
