摘要
目的:探讨丹参酮ⅡA对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱发的帕金森病(PD)模型小鼠中脑黑质区多巴胺能神经元损伤的影响,阐明其对多巴胺能神经元的保护作用及其可能机制。方法:将60只C57BL/6N小鼠随机分为对照组、PD模型组和丹参酮ⅡA组,每组20只。PD模型组和丹参酮ⅡA小鼠采用MPTP制备PD小鼠模型,丹参酮ⅡA组制备PD模型后腹腔注射丹参酮ⅡA,观察各组小鼠行为学表现,采用免疫组织化学、免疫蛋白印迹和免疫荧光双标法检测各组小鼠中脑黑质区酪氨酸羟化酶(TH)、整合素超家族成员Ⅰ型跨膜蛋白(cd11b)、烟酰胺腺嘌呤二核苷酸(NADPH)氧化酶、诱导型一氧化氮合酶(iNOS)阳性细胞数和蛋白表达水平。结果:与对照组比较,PD模型组小鼠出现典型的PD样症状;PD模型组小鼠中脑黑质区TH阳性神经元数和蛋白表达水平较对照组减少约45%和50%,cd11b、p47-phox和iNOS阳性细胞数和蛋白表达水平增加(P<0.01)。与PD模型组比较,丹参酮ⅡA组小鼠PD样症状减轻,黑质区TH阳性神经元数量和蛋白表达水平明显升高(P<0.01),cd11b、p47-phox和iNOS阳性细胞数明显减少(P<0.01),蛋白表达水平明显降低(P<0.01)。结论:丹参酮ⅡA可在一定程度上阻抑MPTP诱导的PD模型小鼠中脑黑质区多巴胺能神经元的丢失,其神经保护作用机制可能与抑制小胶质细胞激活、NADPH氧化酶和iNOS表达有关。
Objective To discuss the influence of eldepryl on the expressions of glial fibrillary acidic protein （GFAP） and cd11b in substantia nigra and striatum in the rats with Parkinson’s disease （PD）,and to clarify the regulatory role of eldepryl in the gliacytes.Methods 72 SD rats were randomly divided into control group,PD model group and eldepryl group,and each group was divided randomly into 4 d and 8 d subgroups （n=12）after the success of model preparation.The PD rat models were established by injecting rotenone in subcutaneous.The number of GFAP and cd11b positive cells and the expressions of GFAP and cd11b were detected by immunohistochemistry and Western blotting method.Results The GFAP and cd11b positive cells were all in a resting state in control group, the GFAP-positive cell body was slender and irregular and had elongated protrusions;the cd1 1 b-positive cell body was small and branch-like,and it had more slender protrusions.The GFAP and cd11b positive cells were all in a active state in model group, the GFAP-positive cell body was hypertrophy, the proj ections increased thickening;the cd1 1 b-positive cell body was more bigger, the proj ections were shorter and thicker, and the number was increased.Compared with model group, the GFAP-positive cell body and protrusions were more slender, the CD11b-positive cell body was more smaller,the projections were more slender,and the number was decreased in eldepryl group.There were a small amount of expression of GFAP and cd11b positive cells in substantia nigra and striatum in the rats in control group,and there was no significant difference between 8 d group and 4 d group（P〈0.05）. The number of GFAP and cd11b positive cells and the protein expression levels were significantly increased in model group compared with control group（P〈0.01）;there was more expression in 8 d group compared with 4 d group,but there was no significant difference（P〈0.05）.The number of GFAP and cd11b positive cells and the protein expression levels in eldepryl
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2014年第5期947-952,I0002,共7页
Journal of Jilin University：Medicine Edition
基金
河北省科技厅自然科学基金资助课题(C2004000689)
河北省科技厅博士基金资助课题(05547008D-4)
河北省科技厅科学技术与社会发展计划项目资助课题(04276135)
