摘要
目的了解辽宁省A组轮状病毒的感染情况,为轮状病毒的预防控制提供科学依据。方法采集辽宁省沈阳、大连、丹东、阜新4个市门诊及住院疑似病毒性腹泻患者粪便标本135份,采用酶联免疫吸附试验(ELISA)检测轮状病毒抗原,阳性标本用逆转录-聚合酶链反应(RT-PCR)扩增A组轮状病毒VP7基因和VP4基因,RT-PCR产物进行核苷酸碱基序列的测定和比对,并构建VP7基因遗传进化树。结果 135份粪便标本中,共检测出A组轮状病毒阳性标本21份;A组轮状病毒G基因分型:G9型15株,G3型2株,G2型1株,G1型1株,未分型2株;P基因型分型:P[8]型20株,P[4]型1株;G/P基因型组合以G9P[8]为主共15株,G3P[8]2株,G1P[8]1株,G2P[4]1株,G/P[8]2株。结论辽宁省首次检出G9型A组轮状病毒,主要流行G/P基因型组合为G9P[8]。
Objective To investigate the prevalence of group A rotavirus in Liaoning province and to provide evidences for control of rotavirus infection. Methods A totol of 135 stool specimens were collected from suspected virus diarrhea patients and group A rotavirus was detected with enzyme-linked immunosorbent assay（ELISA）. VP7 gene and VP4 gene of group A rotavirus were amplified by reverse transcription polymerase chain reaction（RT-PCR）. The PCR products were sequenced and compared and phylogenetic tree was constructed according to VP7 gene of A rotavirus. Results Among the specimens,21 were group A rotavirus. Nucleotide sequence comparison and phylogenetic analysis identified15 cases of G9 genotype of group A rotavirus,2 cases of G3,one case of G2,one case of G1,and 2 cases of unidentified genotype. P genotype results of group A rotavirus showed 20 cases of P[8]and one case of P[4]. There were totally 15 isolates of G9P[8]A rotavirus,2 isolates of G3P[8],2 isolates of G/P[8],and one isolate of G2P[4]. Conclusion G9 genotype of group A rotavirus was detected for the first time and G9P[8]isolates were dominant in Liaoning province.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2015年第4期476-478,共3页
Chinese Journal of Public Health
基金
国家科技重大专项(2012ZX10004209)
