摘要
目的对2001至2005年上海地区住院患儿A组轮状病毒分子流行病学进行研究,为今后轮状病毒疫苗的研制和应用提供基础数据和理论依据。方法从复旦大学附属儿科医院2001至2005年住院的腹泻婴幼儿1450份轮状病毒抗原阳性粪便标本中用机械抽样的方法随机抽取363份,建立套式RT-PCR的方法,从粪便中检测轮状病毒基因并进行G和P型别的分型。结果轮状病毒腹泻的主要流行季节是10~12月份,2岁以内的患儿占全部患儿总数的79.9%(290/363)。G1型所占比例在2001年最多为28.8%(25/87),以后逐年下降至2004年的1.7%(1/60),但2005年又回升至7.0%(4/57)。G3型在5年间均是最主要的流行型别。存在但少见的流行型别是G2和G4型,2001和2002年分别为14.9%(13/87)和5.0%(5/100)。每年的主要流行株均为P[8],不同年份尚流行少量的P[4]和P[6]型。G3P[8]成为2001至2005年上海地区的主要轮状病毒流行株,其次的流行型别包括2001和2003年份的G1P[8],2002年的G3、P混合型,2004至2005年的G3、P未知型。结论2001至2005年上海地区轮状病毒流行型别显示了一定的变化规律,G3、P[8]及其组合G3P[8]是5年间上海地区的主要轮状病毒流行株,不同年份尚有一些其他已知和未知型别的流行。
Objective Group A rotavirus is the most important pathogen leading to dehydrating diarrhea in young children in worldwide scope. This study aimed to study the epidemiology of rotaviruses in hospitalized children in Shanghai, China during the years 2001-2005. The information will provide important data and theoretical bases for study and production of rotavirus vaccine. Methods Systematic sampling was applied for collection of 363 fecal samples from hospitalized children with rotavirns diarrhea in children~ hospital, Fudan University. ELISA was used to detect rotavirus in stool samples, and then nested RT-PCR was applied for determination of rotavirus genotypes. Results The largest proportion of children with rotavirus diarrhea was in the 6- 11 month age group, followed by the 12 -23 months age group（29. 8% and 26.7% respectively）. 79.9% of children with rotavirus diarrhea were younger than 2 years of age. 97 rotavirus positive samples were detected in November, accounting for 26.7%, the other predominant months were October （49 samples, 13.1% ） and December（54 samples, 14. 9% ）. 62. 3% of children with rotavirus diarrhea were detected during October to January of the next year. Among G genotypes of rotavirus in 2001, G3（47. 1% ） was the most predominant, followed by G1 at 28. 8%, G4 at 8. 0% and G2 at 6. 9%. In the year of 2002, G3 was accounted for 62. 0%, followed by G1 （21.0%） and type frequency was 5.0% for G2 and G4 together. G3 increased to 74. 6% in 2003 and G1 was the secondly prevailing type but decreased to 18.6%. All the other samples in 2003 could not be typed. During the year of 2004, G3 type continued to increase to 85% and G1 dropped to 1.7%. G3 accounted for 87.7% in 2005 but G1 turned up to 7%. Several mixed infection of G types were detected in 2001,2002 and 2005 such as combination of any two types of G1, G2 and （;3. Only one G9 was detected in 2001 in all the 363 samples. The dominant P type in 2001 was P[8] （79. 3% ）, followed by P[ 4 ] （6. 9% ） and P[ 6 ] （2.
出处
《中国循证儿科杂志》
CSCD
2007年第2期102-107,共6页
Chinese Journal of Evidence Based Pediatrics
