摘要
1991年秋冬季收集了北京等我国四个地区婴幼儿腹泻患儿粪便标本,通过聚丙烯酰胺凝胶电泳(PAGE)发现一些标本中有不同轮状病毒的混合感染。应用套式多聚酶链反应(套式PCR),确定了7例混合感染轮状病毒的VP7血清型别,并用Northern印迹杂交法证实。该方法用于鉴别混合感染毒株的血清型别,不仅敏感、特异,而且节省时间和材料。
Stool specimens from children with diarrhea were collected from four different areas of China in autumn and winter of 1991. The electrophoretic patterns of viral RNA on polyacrylamide gel suggested coinfection of rotaviruses in some of the specimens. The serotypes of rotavirus coinfections were determined by nested polymerase chain reaction,and were further confirmed by Northern blot hybridization.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
1995年第2期105-109,共5页
Chinese Journal of Microbiology and Immunology
