摘要
将昆明鼠随机分为A、B两组,分别肌肉注射含有猪轮状病毒(RV)JL94株VP7基因的重组真核表达质粒pcDNA-VP7和真核表达载体pcDNA3.1(+),每只鼠100μg·(100μL)-1,共免疫3次,每次间隔2周。首次免疫第0,14,21,28,35,39,47,54天采血分离血清用ELISA法检测抗体动态变化。A组小鼠血清在首免后第14天即可检出阳性抗体(P/N≥2.0)。
The Kunming mice used for nucleic acid immunization were divided into two groups. Group A was injected with 100 μg of the recombinant plasmid, pcDNA-VP7, which contained VP7 gene of the native JL94 isolate of porcine rotavirus, group B with 100 μg of eukaryotic expression plasmid pcDNA3.1(+)in 100 μL volume via femoral quadriceps intramuscular route, respectively, and the mice in each group were injected three times totally. After primary injection, all mice received booster injection two times with the same dosage and via the same route of primary injection at interval of two weeks. Blood samples collected from mice at 0 d before primary injection and those collected from mice of each group at 14, 21, 28, 35, 39, 47, 54 d, were used for detecting antibody levels by ELISA. The results showed that the antibodies to RV could be detected in the serum of mice from group A from the beginning of 14 days of post-immunization(PI).
出处
《东北农业大学学报》
CAS
CSCD
2004年第4期446-448,共3页
Journal of Northeast Agricultural University
基金
黑龙江省"九五"及"十五"科技攻关计划项目。编号(G99B8-1-1
GC0113510)。
关键词
轮状病毒
VP7基因
核酸免疫
抗体
porcine rotavirus
VP7 gene
nucleic acid immunization
antibody.
