摘要
轮状病毒是引起我国儿童重症腹泻的主要病原。按照WHO轮状病毒监测方案,对2003年间河北省卢龙县开展了以医院和社区为基础的小于5岁儿童轮状病毒腹泻的监测,发现一株新型轮状病毒。该病毒用传统分型引物(G1、G2、G3、G4)扩增不出条带,对其VP7基因全序列测定和分析后确定该毒株为G5型。这是我们在亚洲首次发现的人类G5型轮状病毒。该毒株与猪的G5型C134毒株核苷酸和氨基酸序列同源性分别为88·6%和95·4%,与非洲发现的人的G5型毒株MRC3105核苷酸和氨基酸的同源性为89·9%和94%,与巴西发现的IAL-28毒株核苷酸和氨基酸的同源性为87·2%和93·3%。系统发生树分析表明:卢龙毒株LL36755与其他已经报告的两种猪和一种人类的G5型毒株可能具有相同的起源。这是人轮状病毒G5型首次在亚洲国家发现,而且该毒株可能是由人类轮状病毒与动物轮状病毒毒株自然重组产生。
Rotavirus strains,detected as part of ongoing strain surveillance in Lulong county of China and whose first-round reverse transcription-PCR product could not be genotyped by using conventional genotyping primers, were subjected to sequence analysis for strain characterization, We detected for the first time in Asia a human rotavirus with G5 specificity. The Lulong G5 strain named LL36755 had a long electrophoretic pattern. The VP7 gene shared a higher nucleotide and amino acid homology with the porcine G5 strain C134 （88, 6% and 95.2%, respectively） and the human G5 strain MCR3105 （89.9% and 94% respectively ）. Phylogenetic analysis showed Lulong strain LL36755 clustered together in the same lineage as two other reported porcine G5 rotavirus strains and one reported human G5 rotavirus strain. The Lulong G5 rotavirus strain, the first to be reported in humans of Asia, may be a natural reassortant between animal and human rotavirus strains.
出处
《病毒学报》
CAS
CSCD
北大核心
2007年第2期96-102,共7页
Chinese Journal of Virology
