摘要
目的 用丙型肝炎病毒（HCV）核心抗原酶联免疫吸附技术（HCV-cAg ELIsA）检测抗-HCV ELISA阳性无偿献血血标本,了解丙型肝炎病毒核心抗原筛查献血者应用价值.方法 将225份抗-HCV ELISA检测阳性标本分别用HCV-cAgELISA和HCV RT-PCR检测.结果 225份抗-HCVELISA检测不合格标本HCV-cAgELISA和HCV RT-PCR阳性率分别为39.1％和38.7％,阳性率差异无统计学意义（P＞0.05）.复检双试剂均阳性、初或复检单试剂阳性和S/CO值在灰区标本抗-HCVELISA的假阳性率分别为31.4％、90.4％和83.0％,总假阳性率为61.3％.HCV-cAgELISA的假阳性率分别为1.4％、0和0,总假阳性率为1.1％.HCV cAg ELISA检测不同样本假阳性率为0-1.4％明显低于抗-HCV ELISA检测的阳性率31.4％ -90.4％（P＜0.01）.结论 HCV-cAg ELISA敏感性与HCV RT-PCR类似,假阳性率明显低于抗-HCV ELISA,HCV-cAg ELISA具有简便、快速、可靠优点,较适用于安全输血中献血者的血液筛检.
Objective To detect anti-hepatitis C virus enzyme-linked immunoassay （anti-HCV ELISA） positive blood specimens by HCV core antigen ELISA（HCV-cAgELISA）,and to investigate the value of detecting of HCV-cAg for screening blood donor.Methods All anti-HCV ELISA positive specimens were identified by using HCV-cAgELISA and HCV RT-PCR methods.Results In 225 anti-HCV positive specimens,the positive rates of HCV-cAgELISA and HCV RT-PCR were 39.1% and 38.7%,respectively,the positive rates were no significant difference.Among anti-HCV screening unqualified samples,the false positive rates of anti-HCV ELISA in both positive,single positive,and grey area were 31.4%,90.4% and 83.0%,respectively.The total false positive rate was 61.3%.The false positive rate of HCV-cAgELISA in both positive,single positive,and grey area were 1.4%,0 and 0,respectively.The total false positive rate was 1.1%.The false positive rate of HCV-cAgELISA was significantly lower antiHCV ELISA （P 〈 0.01）.Conclusion Sensitivity of HCVcAg ELISA is silllilar to HCV RT-PCR （P 〉 0.05）.The total false positive rate of HCVcAg ELISA was significantly lower anti-HCV ELISA （P 〈 0.01）.HCVcAg ELISA is a simple,fast,and reliable memod to screen donor with HCV infection in blood transfusion medicine.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2015年第1期68-70,共3页
Chinese Journal of Experimental and Clinical Virology
基金
衢州市科技计划项目（2013J063）
