摘要
目的研究积雪草苷对心肌梗死(心梗)大鼠左室心肌纤维化和心肌组织转化生长因子-β1(transforming growth factor-β1,TGF-β1)、胶原Ⅰ(collagenⅠ,ColⅠ)和胶原Ⅲ(collagenⅢ,ColⅢ)表达的影响。方法结扎雄性SD大鼠左冠状动脉前降支建立心梗模型,随机分为假手术组(Sham组)、假手术+积雪草苷组(Sham+Asiaticoside组)、心梗模型组(MI组)和心梗模型+积雪草苷组(MI+Asiaticoside组)。造模当天给药,早晚各1次,2周后分别测定各组大鼠心脏左室重量与体重之比、左室梗死面积;免疫组织化学(免疫组化)检测心肌组织非梗死区TGF-β1、ColⅠ、ColⅢ的表达。计量资料采用t检验,P<0.05为差异有统计学意义。结果 MI组左室梗死面积为(24.37±5.69)%,MI+Asiaticoside组左室梗死面积为(25.89±6.27)%,差异无统计学意义(P>0.05);MI组左室重/体重为(3.46±0.26)mg/g,MI+Asiaticoside组左室重/体重为(2.53±0.35)mg/g,差异有统计学意义(P<0.05);免疫组化染色结果显示,与MI组相比[(8.34±0.62)、(37.66±2.21)、(12.39±1.37)μm2],MI+Asiaticoside组非梗死区心肌TGF-β1、ColⅠ、ColⅢ的表达[(5.97±0.78)、(17.90±1.36)、(8.14±1.23)μm2]明显降低,差异均有统计学意义(均P<0.05)。心梗大鼠非梗死区心肌组织ColⅠ、ColⅢ表达与心肌组织TGF-β1的表达正相关。结论积雪草苷可减少心梗大鼠非梗死区TGF-β1的表达,从而减弱非梗死区心肌组织ColⅠ、ColⅢ异常合成与沉积,减轻心梗后左室心肌纤维化程度,延缓左室重构的发展。
Objective To study the effect of Asiaticoside on myocardial fibrosis and the expression of transforming growth fac- tor-β1（TGF-β1） and collagen Ⅰ （Col Ⅰ） and collagen Ⅲ （Col Ⅲ） of left ventricular non-infarcted myocardium in rats after myocardial infarction.Methods MI model was established in male SD rats by ligation of left anterior descending coronary artery.The rats were randomly divided into four groups：sham-operated group（Sham group）,sham-operated＋Asiaticoside group（Sham＋Asiaticoside group）,MI model group（MI group） and Asiaticoside treatment group（MI＋Asiaticoside group） after myocardial infarction.After 2 weeks of treatment,the cardiac function was assessed by the ratio of left ventricular weight and body weight,infarct size.The proteins of myocardial TGF-β1 and Col I and Col m of non-infarcted myocardium were determined through immunohistochemical methods. Groups were compared using t test,P〈0.05 as statistically significant difference.Results Left ventricular infarct size of MI model group was （24.37 ± 5.69）%,and left ventricular infarct size of Asiaticoside treatment group was （25.89± 6.27）%,the difference was not statistically significant（P〉0.05）.The ratio of left ventricular weight and body weight in MI model group was （3.46 ± 0.26）mg/g, and the ratio of left ventricular weight and body weight in MI＋Asiaticoside treatment group was （2.53 ± 0.35）mg/g,the difference was statistically significant（P〈0.05）.Immunostained TGF-131,Col I and Col Ⅲ of non-infarcted myocardium were obviously lower in the group. MI＋Asiaticoside than those in the MI model group[（5.97 ± 0.78）,（17.90 ± 1.36）,（8.14 ±1.23）,（8.34 ± 0.62）,（37.66± 2.21）, （12.39 ± 1.37）μm2]（all P〈0.05）.The expression of Col Ⅰ ,Col Ⅲ and the expression of TGF-131 in rats after myocardial infarction showed a significant positive correlation.Conclusion Asiaticoside can inhibit myocardial fibrosis through decreasing the expression of T
出处
《社区医学杂志》
2015年第5期1-5,共5页
Journal Of Community Medicine
基金
河南省南阳市2012年科技发展计划(2012GG066)
