摘要
目的:研究番茄红素(lycopene)对人慢性髓系白血病K562细胞凋亡的影响,探讨其凋亡的作用机制。方法:用不同浓度(0,20,40,60μmol·L-1)的番茄红素作用K562细胞24,48,72 h,0μmol·L-1番茄红素为空白组。采用MTT法检测不同浓度的番茄红素作用24,48,72 h时K562细胞增殖抑制率;Annexin V/PI双染色法检测不同浓度的番茄红素作用48 h时K562细胞凋亡率;RT-PCR法及Western blot法检测不同浓度的番茄红素作用48 h时K562细胞中B细胞淋巴瘤-2(Bcl-2),半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)mRNA及蛋白的表达。结果:与空白组比较,细胞增殖抑制率随番茄红素浓度升高而明显升高,细胞凋亡率逐渐明显增加,随番茄红素浓度明显升高,Bcl-2 mRNA及蛋白的表达明显降低,Caspase-3mRNA及蛋白的表达明显升高,均具有统计学差异(P<0.05)。结论:番茄红素诱导人慢性髓系白血病K562细胞凋亡,其凋亡机制与下调Bcl-2及上调Caspase-3表达有关。
Objective： To investigate the induced apoptotic effects and its mechanism of lycopene on human leukemia cell line K562 cells. Method： K562 cells were treated with different concentrations of lycopene （0, 20, 40, 60 μmol·L^-1）. Cell proliferation in K562 was observed by MTT. Cell apoptosis was inspected by Annexin V/PI double staining method. The expressions of Bcl-2 and Caspase-3 were determined using RT-PCR and Western blot. Result： MTT and Annexin V/PI showed that the rates of proliferation inhibition and apoptosis were positively associated with the concentrations of lycopene （P 〈 0.05）. RT-PCR and Western blot indicated that the expression of Bcl-2 gene decreased negatively, but the expression of Caspase-3 gene decreased positively associated with concentration of lycopene （P 〈 0.05）. Conclusion： Lycopene could induce the apoptosis of K562 cells, which may be associated with lowering expressions of Bcl-2 and enhancing caspase-3 activity.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第7期133-136,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅科技基金项目(黔科合SY字20123138号)
贵阳市科技局科技基金项目(筑科合201210334号
筑科合201210336号)
