摘要
目的研究EPO治疗干预过氧化氢诱导人RPE细胞氧化损伤的作用机制。方法 ARPE-19细胞株随机分为正常对照组、过氧化氢诱导组(模型组)和EPO治疗干预组。MTT法检测细胞活力变化,免疫组织化学法检测各组细胞Caspase-9蛋白的表达变化。结果 MTT法检测结果显示,过氧化氢可以显著降低细胞的活性,与对照组相比较,t=11.8692,P<0.01。EPO干预组与模型组比较,10IU/mLEPO干预组、20IU/mLEPO干预组、40IU/mLEPO干预组细胞活力逐渐升高,t值分别为5.6569、5.7056、9.4299(P<0.01)。模型组Caspases-9呈强阳性表达,与正常组比较,表达升高,t=147.5805,P<0.01。经不同浓度的EPO干预,Caspase-9的表达不同程度的减弱,与模型组比较,10IU/mLEPO干预组、20IU/mLEPO干预组、40IU/mLEPO干预组表达逐渐下降,t值分别为18.7409、61.8718、54.9153,P<0.01。结论过氧化氢诱导RPE细胞氧化损伤细胞活力下降,EPO可以保护过氧化氢诱导的RPE细胞氧化损伤,其机制可能与抑制凋亡,减弱凋亡诱导因子Caspase-9的表达有关。
Objective To study of erythropoietin therapy intervention induced by hydrogen peroxide in human retinal pigment epithelial（RPE） ceils from oxidative damage mechanism. Methods ARPE-19 cell lines were randomly divided into normal control group,hydrogen peroxide induced group（model group） and EPO treatment group. MTT detected cell viability, immunohistochemical method to detect the expression of Caspase-9 protein. Results MTT examination result show, hydrogen peroxide can significantly reduce the activity of RPE cells, compared with control group, t=l 1.869 2,P 〈 O.01.EPO intervention group compared with the model group,lO IU/mL EPO intervention group,20 IU/mL EPO intervention group,40 IU/mL EPO intervention group cell viability gradually increased,t values were：5 656 9,5.705 6,9.429 9,P 〈 0.01. Model group Caspase-9 showed a strong positive expression, compared with the normal group,expression is elevated,t=147.580 5,P 〈 0.01. With different concentration of EPO intervention,Caspase-9 expression decreased. Compared with the model group, 10 IU/MLEPO intervention group, 20 IU/mL EPO intervention group,40 IU/mL EPO intervention group expression gradually declined,t values were：18.740 9,61.871 8, 54.915 3,P 〈 0.01. Conclusion Hydrogen peroxide induced oxidative damage in RPE cells by decreasing cell viability,EPO can protect RPE cell oxidative damage induced by hydrogen peroxide.Its mechanism may be related to inhibition of apoptosis and reduction of Caspase-9 expression.
出处
《中国医药科学》
2011年第24期32-33,42,共3页
China Medicine And Pharmacy
基金
国家自然科学基金资助(30572010)
山东省科学技术奖(科技进步奖
JB2009-3-241-3)
