摘要
目的:体外观察银杏内酯B对H_2O_2诱导的骨髓间充质干细胞(BMSCc)凋亡的影响。方法:(1)采用全骨髓贴壁法体外分离、培养骨髓间充质干细胞,在倒置显微镜下观察BMSCs形态变化。(2)实验随机分为空白对照组和银杏内酯B组(10、25、50、100、200、400μmol·L^(-1)),采用MTT法检测不同浓度的银杏内酯B作用24h对H_2O_2诱导损伤的细胞活性改变的作用,观察银杏内酯B对细胞影响的量效关系。(3)采用TUNEL染色法检测不同分组下BMSCs的凋亡情况。结果:(1)采用全骨髓贴壁法分离、培养得到数量较多,形态均一稳定的BMSCs。(2) MTT结果表明与对照组相比,银杏内酯B预处理BMSCs能显著提高细胞的活性(P <0. 05)。(3) TUNEL染色法实验结果表明,与对照组相比,银杏内酯B(50、100μmol·L^(-1))能降低H_2O_2诱导的BMSCs细胞的凋亡率(P <0. 01或P <0. 05)。结论:银杏内酯B能抑制H_2O_2诱导的BMCSs凋亡,促进细胞存活。
Objective： To observe the effect of Ginkgolide B （GB） on hydrogen peroxide-induced rat bone marrow-mesenchymal stem cells （BMSCs） apoptosis. Methods： （1）Isolate and culture the BMSCs by the whole bone marrow adherent method, the morphological changes were observed under the inverted microscope. （2）The experiment was randomly divided into blank control group and ginkgolide B group（10,25,50,100,200,400μmol·L -1 ）,MTT method was used to detect the cell viability after pretreatment with different concentrations of GB on hydrogen peroxide-induced BMSCs,and observed the dose-effect relationship of GB. （3）The terminal deoxynucleotidyl transferase dUTP nick end labeling （TUNEL） assay was used to detect the apoptosis of different groups of BMSCs. Results： （1）The whole bone marrow adherent culture method could obtain considerable amount of morphological stable rat BMSCs in vitro. （2）The MTT assay showed that compared with control group, incubated with GB could improve BMSCs viability significantly （ P 〈0.05）. （3）The TUNEL assay showed that compared with control group, the GB pretreatment （50,100μmol·L -1 ） reduce the apoptosis rape of hydrogen peroxide-induced BMSCs （ P 〈0.01 or P 〈0.05）. Conclusion： GB protects hydrogen peroxide-induced apoptosis on BMSCs, and protect the BMSCs viability.
作者
鲍庆
BAO Qing;WANG Jun(Huzhou Third People＇s Hospital,Zhejiang Province 313000)
出处
《医学理论与实践》
2018年第19期2849-2851,共3页
The Journal of Medical Theory and Practice
关键词
银杏内酯B
H2O2
骨髓间充质干细胞
凋亡
Ginkgolide B
Hydrogen peroxide
Bone marrow mesenchymeal stem cells
Apoptosis
