摘要
品质育种已成为我国水稻育种的重要目标之一,用分子标记选择优良米质的基因型是重要的品质改良方法。本研究以低直链淀粉含量（AC）的籼型恢复系品种R122、R527和R998作为优质基因的供体,以产量配合力较强的三系籼型恢复系R225为受体轮回亲本,通过杂交、多次回交和自交结合分子标记辅助选择,对高AC的恢复系R225进行回交改良,同时利用RM190分子标记对控制AC值的基因型进行选择。结果表明,将供体材料控制直链淀粉的Wx基因导入到恢复系R225中,后代株系的改良材料直链淀粉含量有了显著降低,受体亲本的直链淀粉含量由25.4%下降到11.6%~23.5%;同时对回交轮回亲本进行背景选择,SSR标记分析表明,改良株系的遗传背景回复率达81.1%~92.6%。通过标记辅助选择获得的改良材料为三系杂交稻恢复系的培育提供了优良亲本。
Quality breeding has become one of the important objectives in rice breeding in China.Selection of genotypes with good quality by molecular marker is an important method of quality improvement.To lower the high amylose content of an elite indica rice restorer line R225,three indica varieties（R122,R998 and R527）with low amylose content were used as donor and backcrossed with recurrent parent R225 respectively,and their backcrosses were identified using a molecular marker RM190.Analysis of genotypes and AC of improved lines revealed that the AC of new lines with new Wx gene were degrated from 25.4% to 11.6%~23.5%.Background SSR analysis showed that the recovery rate of the selected lines reached 81.1%~92.6%.The improved lines can be used as candidate parental lines for breeding of hybrid rice.
出处
《中国稻米》
北大核心
2015年第2期44-47,共4页
China Rice
基金
江西省农业科学院青年基金项目(NXCYTY-01)
江西省重大项目"超级稻品种的培育与示范推广"子项目(20114ABF03105)
江西省科技支撑计划项目"超级稻种质创新与组合选育"(2009BNA03600)
关键词
标记辅助选择
直链淀粉含量
三系杂交稻
恢复系
marker-assisted selection（MAS）
intermediate amylase content
hybrid rice
restorer line
