摘要
目的:探究用PCR方法检测食品中金黄色葡萄球菌(Staphylococcus aureus)、沙门氏菌(Salmonella spp.)及志贺氏菌(Shigella spp.)的检测灵敏度,建立快速检测3种食源性致病菌的多重PCR方法。方法:分别依据金黄色葡萄球菌的fem A、沙门氏菌的hil A基因及志贺氏菌的ipa H基因设计3对特异性引物,对3对引物进行多重PCR反应体系构建与优化。结果:建立的多重PCR方法具有灵敏度高、特异性强、方便快捷的优点。结论:研究结果为上述3种食源性致病菌快速检测试剂盒的研发及在食品安全检测工作中的应用提供了重要技术保障。
Objective： To determination the detection sensitivity of Staphylococcus aureus, Salmonella spp. and Shigella spp. in food by PCR, and then to establish a multiplex PCR method for synchronous rapid detection of three foodborne bacterial pathogens. Methods： Three pairs of specific primers were designed according to the specific gene femA of Staphylococcus aureus, gene hilA of Salmonella spp, and ipaH of Shigella spp, and a multiplex PCR reaction system for these three foodborne bacterial pathogens was established and optimized. Results： The multiplex PCR method was confirmed to have many advantages, such as high sensitivity, strong specificity, convenient and efficient to operation. Conclusions： This method provided a key technical support for developing a kit for synchronous rapid detection of the three species of foodborne bacterial pathogens, and which could be easily applied in present food safety testing.
出处
《食品科技》
CAS
北大核心
2015年第3期324-329,共6页
Food Science and Technology
基金
“十二五”国家科技支撑计划项目(2012BAK08B04-01)
中粮集团应用基础研究项目(2012-C2-F014)
