摘要
目的通过探讨Foxo1-KLF2-S1P1在MG患者胸腺的表达,分析其对胸腺T细胞输出的影响。方法取MG患者及对照组胸腺组织,提取总RNA,通过荧光定量RT-PCR检测Foxo1、KLF2、S1P1及CD62L、CCR7、CD69的表达;制作胸腺组织切片,通过免疫组化了解Foxo1、S1P1、CD62L、CD69、CCR7在胸腺组织的分布与表达。结果免疫组化显示Foxo1、S1P1、CCR7在MG患者及对照组胸腺都有表达,主要分布在髓质区,MG患者胸腺组织髓质区扩大,Foxo1、S1P1、CCR7表达显著高于对照组;荧光定量PCR结果显示MG胸腺组织Foxo1、KLF2、S1P1、CCR7、CD69的m RNA的水平表达显著增高(分别是5.36±0.728、1.43±2.71、6.1±1.033、5.0±0.932、4.97±1.112,与对照组相比P<0.05)。结论 MG患者Foxo1-KLF2-S1P1高表达可能是MG患者胸腺细胞的异常输出的原因。
This study aims to explore the roles of Foxo1-KLF2 and S1P1 in T cells output of myasthenia gravis （MG） thymus by analyzing the expression of Foxo1-KLF2 and S1P1 in thymus of klG patients. Total RNA extracted from the thymus tissue of MG patients and control people were collected, then the mRNA expression levels of Foxol, KLF2, S1P1, CD62L, CCR7, and CD69 were examined by real-time PCR. At the same time, Paraffin-embedded sections of thymus from MG patients and control people were made and stained with HE, then the protein expression and distribution of Foxo1, S1P1, CD62L, CCR7, and CD69 were checked by immunohistochemistry method in these Paraffin-embedded sections. The results of real-time PCR showed that the mRNA expression of Foxo1, KLF2, S1P1, CCR7, and CD69 in thymus tissue of MG increased significantly as compared with those in thymus tissue of control people （P〈 0.05）; the results of immunohistochemistry showed that although Foxol, S1P1 and CCR7, mainly distributed in the medulla area in thymus, expressed not only in the thymus tissue of MG patients but also control people, the medulla area of thymus tissue in MG patient was expanded than that in control people, and the protein expressions of Foxo1, S1P1 and CCR7 were significantly higher in thymus tissue from MG patients than those from control people （P〈 0.05）. Taken together, high expression of Foxo1-KLF2-S1P1 in thymus could be the major reason for unusual output of T cells in MG patients.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2015年第2期181-184,共4页
Immunological Journal
基金
国家自然科学基金(81471545
81172874)
