摘要
目的对2013—2014年度连云港市流行性感冒的病原学监测结果进行分析,了解连云港市流感流行动态,探索流行规律,为流感防治提供科学依据。方法采集流感样病例(ILI)的鼻咽拭子标本,用狗肾细胞(MDCK)进行病毒分离,采用血凝抑制方法(HI)进行流感病毒型别鉴定。结果 2013年4月—2014年3月共检测流感哨点医院ILI鼻咽拭子标本970份,分离到流感病毒44株,阳性分离率为4.54%,经分型鉴定新甲H1N1亚型35株(79.55%),A型H3亚型5株(11.36%),B型Yamagata亚型4株(9.10%)。结论 2013年流行的优势毒株为新甲H1N1亚型,2014年流行的优势毒株为B型Yamagata亚型,每年的3、4月份会出现流感亚型的转变。
Objective To understand the influenza epidemic dynamic and rules in Lianyungang City from 2013 to2014 so as to provide the evidence for improving the prevention from influenza.Methods The nasopharyngeal swab samples from influenza-like illness（ILI） were collected.The viruses were isolated with MDCK cells and identified with HI.Results A total of 44 influenza strains were obtained from 970 specimens,and the positive rate was 4.54%.Thirty-five of them were identified as swine-origin influenza A（HlNl） virus,5 of them were subtype A（H3） and 4 of them were influenza B virus.Conclusion The main type is swine-origin influenza A（HlNl） virus in 2013.Influenza B virus predominates in2014,and changes every year around March in influenza subtypes.
出处
《中国校医》
2015年第2期115-117,共3页
Chinese Journal of School Doctor
关键词
正黏病毒科/分离和提纯
血凝抑制试验
马丁达比狗肾上皮细胞
Orthomyxoviridae/isolation and purification
Hemagglutination Inhibition Tests
Madin Darby Canine Kidney Cells
