摘要
目的探讨姜黄素联合顺铂对非小细胞肺癌细胞A549放射增敏的影响。方法采用MTT法观察不同浓度姜黄素（10、20、50、100、200μmol/L）和顺铂（1、2、5、10、20 mg/L）作用24、48及72 h后的细胞存活率,根据实验设计分为单纯照射（R）组、姜黄素＋照射（C＋R）组、顺铂＋照射（P＋R）组及姜黄素＋顺铂＋照射（C＋P＋R）组,采用克隆形成实验检测4组经不同剂量X线（0、2、4、6、8、10 Gy）照射后的存活分数（SF）并通过单击多靶模型拟合细胞存活曲线计算增敏比（SER）,分别采用人工划痕、Transwell试验及Western blotting检测以上4组处理24 h后的细胞迁移、侵袭及表皮生长因子受体（EGFR）的蛋白水平。结果随姜黄素（10-200μmol/L范围内）和顺铂（1-20 mg/L范围内）浓度增加,A549细胞的细胞存活率降低,抑制效应呈浓度和时间依赖方式,差异有统计学意义（P〈0.05）;C＋P＋R组在照射剂量为2-10 Gy时的细胞SF均低于其余3组,而C＋R组在4-10 Gy,P＋R组在2-10 Gy时的细胞SF均低于R组,差异有统计学意义（P〈0.05）,C＋R组、P＋R组及C＋P＋R组相对于R组的SER依次为1.24、1.31和1.96;C＋P＋R组的迁移距离、穿膜细胞数量及EGFR蛋白水平均低于其余3组,而C＋R组和P＋R组的以上指标亦低于R组,以上差异均有统计学意义（P〈0.05）。结论姜黄素联合顺铂可抑制A549细胞增殖并具有放射增敏作用,同时抑制其迁移和侵袭,可能与EGFR相关信号通路受抑制有关。
Objective To explore the radiosensitization effect of curcumin plus cisplatin on non-small cell lung cancer A549 cells. Methods Cell viability at 24,48 and 72 h after treatment with different concentrations（ 10,20,50,100,200 μmol / L） of curcumin or（ 1,2,5,10,20 mg / L） of cisplatin were determined by MTT. According to the experimental protocol,the below experiments were carried out in irradiation（ R） group,curcumin ＋ irradiation（ C ＋ R） group,cisplatin ＋ irradiation（ P ＋ R） group and curcumin ＋ cisplatin ＋ irradiation（ C ＋ P ＋ R） group. The colony formation assay was employed to observe the surviving fraction（ SF） of above four groups after X-ray irradiation of 0,2,4,6,8,10 Gy. The artificial scratch,Transwell test and Western blotting were employed to detect the cell migration,invasion and protein level of epidermal growth factor receptor（ EGFR） at 24 h after treatment in four groups. Results The cell viability of A549 cells gradually decreased with the increasing concentration of curcumin ranging from 10 to200 μmol / L and cisplatin ranging from 1 to 20 mg / L in a dose- and time-dependent manner（ P〈0. 05）. The SF of C ＋ P ＋ R group were lower than the remaining 3 groups under the dose of 2-10 Gy（ P〈0. 05）. Compared with the R irradiation group,there was lower SF in C ＋ R group under the dose of 4-10 Gy and P ＋ R group under the dose of 2-10 Gy with significant difference（ P〈0. 05）. Compared with R group,the sensitizing enhancement ratio were 1. 24,1. 31 and 1. 96 in C ＋ R group,P ＋ R group and C ＋ P ＋ R group,respectively.There were lower migration distance,transmembrane cell number and EGFR protein level in C ＋ P ＋ R group versus the remaining 3groups（ P〈0. 05）. Compared with the R group,the above indicators were also lower in C ＋ R group and P ＋ R group under the dose of2-10 Gy with significant difference（ P〈0. 05）. Conclusion Curcumin plus cisplatin can inhibit the proliferation of A549 cells with radios
出处
《临床肿瘤学杂志》
CAS
2015年第1期13-17,共5页
Chinese Clinical Oncology
关键词
姜黄素
顺铂
放疗增敏
表皮生长因子受体
Curcumin
Cisplatin
Radiosensitization effect
Epidermal growth factor receptor
