摘要
目的探讨脑缺血预处理对缺血再灌注损伤(ischemia reperfusion injury,IRI)大鼠外周血内皮祖细胞(endothelial progenitor cells,EPCs)及缺血区脑组织血管新生的影响,并分析EPCs的数量与血管新生关系。方法 SD大鼠108只,随机分为3组:假手术(sham operation,SO)组、大脑中动脉缺血(middle artery occlusion,MCAO)组、脑缺血预处理(brain ischemic preconditioning,BIP)组,各36只,每组再分为缺血再灌注前0 h、缺血再灌注后3 h、24 h、3 d、5 d、7 d 6个时点,每个时点6只大鼠。用Zea Longa评分标准进行神经功能评分。用流式细胞仪计数外周血EPCs数目。用免疫组织化学染色法检测缺血区脑组织新生血管密度。结果 1神经功能缺失评分:再灌注3 h后,BIP组、MCAO组均出现不同程度的神经功能缺失,且BIP组较MCAO组评分低(5 d:1.00±0.63;7 d:1.00±0.63,P<0.05);2外周血EPCs数目:再灌注3 h后,MCAO组外周血EPCs数目开始降低,BIP组逐渐升高,且BIP组较MCAO组明显增加(24 h:0.58±0.07;3 d:0.80±0.10;5 d:0.68±0.05;7 d:0.52±0.03,P<0.01);3新生血管数目:BIP组、MCAO组分别于再灌注后3 d、5 d出现新生血管,且BIP组较MCAO组多(5 d:14.53±3.44;7 d:41.40±5.62,P<0.01);4Pearson相关分析表明,缺血区脑组织新生血管密度与外周血EPCs数量呈正相关(5 d:r=0.855,P<0.01;7 d:r=0.946,P<0.01)。结论 BIP能促进脑IRI大鼠EPCs的动员,有助于增加缺血区脑组织血管新生。
ObjectiveTo investigate the effects of brain ischemic preconditioning（BIP） on peripheral blood EPCs and neovascularization in ischemic brain tissue in rats with cerebral ischemia reperfusion injury（IRI）.MethodsOne hundred and eight male SD rats were randomly divided into three groups： SO group（n=36）, MCAO group（n=36） and BIP group（n=36）. Neurological function assessment was conducted at 0 h before MCAO-reperfusion, 3 h, 24 h and 3 d, 5 d as well as 7 d after MCAO-reperfusion（n=6 for each group in each time point）. Flow cytometry was used to calculate the number of EPCs. Immunohistochemical staining was used to detect the capillary density.Results1 Although neurological deficit scores were significantly decreased in both BIP and MCAO groups after 3 h following MCAO-reperfusion, the scores were much lower in BIP group than in MCAO group（5 d： 1.00±0.63; 7 d： 1.00±0.63, P0.05）. 2 The numbers of EPCs were decreased in MCAO group while was increased in BIP group at 3 h after MCAO-reperfusion. The numbers of EPCs were significantly higher in BIP group than in MCAO group（24 h： 0.58±0.07; 3 d： 0.80±0.10; 5 d： 0.68±0.05; 7 d：0.52±0.03, P0.01）. 3 The new blood vessels could be detected at 3 d in BIP group and 5 d in MCAO group after MCAO-reperfusion. The numbers of new blood vessels were significantly higher in BIP group than MCAO group（5 d：14.53±3.44; 7 d： 41.40±5.62, P〈0.01）. 4 Pearson analysis showed a positive correlation between EPCs and capillary density（5 d： r=0.855, P0.01; 7 d： r=0.946, P0.01）.ConclusionBIP can improve EPCs mobilization and function,which may contribute to neovascularization in the ischemic brain tissue.
出处
《中国神经精神疾病杂志》
CAS
CSCD
北大核心
2015年第1期10-14,共5页
Chinese Journal of Nervous and Mental Diseases
基金
辽宁省科学技术计划项目(编号:2014021070)
关键词
缺血预处理
缺血再灌注损伤
内皮祖细胞
新生血管化
Ischemic preconditioning Ischemia-reperfusion injury Endothelial progenitor cells Neovascularization
