摘要
以条斑紫菜（Pyropia yezoensis）野生型品系（Py-WT,特性：颜色好,生长慢,藻体厚,主要光合色素含量低,耐高温性差）的叶状体作为父本,红色突变型品系（Py-HT,特性：颜色较红,生长快,藻体薄,主要光合色素含量高,具一定的耐高温性）的叶状体作为母本进行种内杂交,从杂交丝状体的子一代叶状体中分离出1个耐高温的重组优良品系（HW-4）。在24℃下培养13 d,父本 Py-WT 和母本 Py-HT 品系的壳孢子存活率分别为18.3%和65.2%,而HW-4品系的壳孢子存活率为77.5%。在25℃下培养13 d,3个品系（Py-WT、Py-HT和HW-4）的壳孢子存活率分别为16.9%、47.1%和67.3%。在高温（24℃和25℃）下培养的HW-4品系的壳孢子存活率分别是Py-WT品系的4.2倍和4.0倍,是Py-HT品系的1.2倍和1.4倍。常温（19℃）培养50 d的壳孢子萌发体,在24℃再继续培养25 d, Py-WT、Py-HT和HW-4品系的叶状体平均体长分别增加了0.6、0.6和2.3倍, HW-4品系的平均体长分别是Py-WT和Py-HT品系的5.9倍和2.6倍；在25℃再培养25 d, Py-WT、Py-HT和HW-4品系的平均体长分别增加了0.3、0.4和1.5倍, HW-4品系的平均体长分别是 Py-WT和 Py-HT品系的5.3倍和2.1倍。另外,两个亲本品系的叶状体在高温（24℃和25℃）下培养10~15 d会卷曲和腐烂,培养至25 d时藻体严重卷曲且大面积腐烂；而HW-4叶状体在高温下培养25 d时藻体仅轻度卷曲,35 d后仍未见腐烂现象。常温（19℃）培养的HW-4叶状体分别在常温（19℃）和高温（24℃和25℃）继续培养15 d,总藻胆蛋白含量分别为76.0、111.1和132.3 mg/g,分别是Py-WT品系的3.1、2.2和2.0倍,是Py-HT品系的1.2、1.1和1.1倍,均显著高于双亲品系（P〈0.05）。3个品系的壳孢子放散量之间无显著性差异（P〉0.05）。上述结果表明,与亲本品系相比, HW-4品系的壳孢子和叶状体的耐高温性均明显提高,叶状体的生长速度和色素含量也大幅度提高,是一个生产适�
Following intraspecific hybridization of the wild-type strain（Py-WT, ♂） and the red-type pigmentation mutant（Py-HT,♀） in Pyropia yezoensis, a high-temperature resistant strain HW-4 was isolated from the recombi-nants. The gametophytic blades of the Py-WT strain have slow growth, thick blades, low major photosynthetic pigment content, and poor heat-resistance characters, while the gametophytic blades of Py-HT have fast growth, thin blades, high major photosynthetic pigment content, and partial high-temperature resistance. After the con-chospores of Py-WT and Py-HT strains were cultured at high temperature （24℃） for 13 days, the survival rates of the conchospores were 18.3%and 65.2%, respectively;however, the survival rate of HW-4 conchospores at 24℃was 77.5%. Furthermore, after the conchospores were cultured at high temperature （25℃, 13 days）, the survival rates of the Py-WT, Py-HT and HW-4 conchospores were 16.9%, 47.1%and 67.3%, respectively. The survival rate of the HW-4 conchospores at 24℃ was 4.2 times that of Py-WT and 1.2 times that of Py-HT;additionally, at 25℃it was 4.0 times that of Py-WT and 1.4 times that of Py-HT. After the conchospore germlings from Py-WT, Py-HT and HW-4 strains （50-day-old, 19℃） were cultured at 24℃ for another 25 days, the mean blade length increased by 1.6, 1.6 and 3.3 times compared with the original lengths, respectively. The mean blade length of the HW-4 strain was 5.9 times that of Py-WT and 2.6 times that of Py-HT. After the 50-day-old conchospore germlings were cultured at 25℃ for another 25 days, the mean length of the blades for Py-WT, Py-HT and HW-4 increased 1.3, 1.4 and 2.5 times, respectively, and the mean length of the blades of the HW-4 strain was 5.3 times that of Py-WT and 2.1 times that of Py-HT. In addition, the gametophytic blades of the parental strains began to curl and rot after 10–15 days in culture at high temperatures（24℃and 25℃）, and rotten seriously after 25 days. However, the HW-4 gametophytic blades cur
出处
《中国水产科学》
CAS
CSCD
北大核心
2015年第1期33-43,共11页
Journal of Fishery Sciences of China
基金
国家863计划项目(2012AA10A411)
国家自然科学基金项目(31072208)
国家海洋局公益专项(201105008
20110-5023)
国家农业科技成果转化资金项目(2013GB2C220537)
农业部公益性专项(200903030)
上海市科委重点科技攻关项目(10391901100)
上海高校水产学一流学科建设项目
关键词
条斑紫菜
种内杂交
嵌合体
耐高温
筛选
特性分析
Pyropia yezoensis
intraspecific hybridization
chimera
high-temperature resistance
selection
char-acterization
