摘要
目的探讨DSA联合CT引导下经皮肝穿刺兔VX2肝癌模型制作的可行性。方法 40只新西兰白兔随机分成A、B两组,每组20只,A组在DSA联合CT引导下将兔VX2肿瘤组织块经皮肝穿刺接种于兔左肝内,B组通过开腹法种植VX2肿瘤组织块,于接种后第2周、4周行全身MR检查,后处死实验兔行原位、异位肿瘤肉眼观察。结果A组建模成功率90%,有1只出现异位种植,未发现腹水及死亡,平均手术时间(17.3±5.2)min;B组建模成功率85%,有3只出现腹水,2只死亡,无异位种植发生,平均手术时间(50.7±11.3)min,,两组手术时间比较差异有统计学意义(P<0.05)。结论 DSA联合CT引导下经皮肝穿刺制作兔VX2肝癌模型方法简单、建模成功率高、手术操作时间短。
Objective To evaluate the establishment method of rabbit liver VX2 tumor model by injecting tumor tissue mass into the liver parenchyma via percutaneous transhepatic approach under DSA and CT guidance. Method Forty New Zealand rabbits were divided into A and B groups randomly with 20 rabbits each. The VX2 tumor tissue mass was implanted into rabbits liver guided through percutaneous transhepatic route guided by DSA and CT in group A and through epigastrium incision method in group B. On the second and third weeks after implantation, MR examination was performed and the rabbits were sacrificed to observe the tumor in situ and ectopia. Result In group A, the success rate of the liver VX2 tumor model was 90% with mean operation time of （ 17.3 ± 5.2） min and there was no episode of ascites and death with the exception of ectopia implantation in one case. In group B, successful establishment rate was 85% with average operation time as long as （17.3 ± 5.2）min and ascites and death were observed in three and two cases respectively. The difference of operation time between two groups was statistically significant （P 〈 0.05）. Conclusion The method of liver VX2 tumor implantation guided by DSA and CT via percutaneous transhepatic approach is simple with higher success rate and shorter operation time for making liver carcinoma model of rabbits.
出处
《实验动物科学》
2014年第6期26-28,35,共4页
Laboratory Animal Science
基金
青岛市科技计划项目(No.08-2-1-3-nsh)
