摘要
目的研究乌骨藤苷晶体对人宫颈腺癌荷瘤裸鼠肿瘤的抑制作用。方法采用人宫颈腺癌接种裸鼠待长出实体瘤,用第三代实体瘤移植裸鼠皮下建立人宫颈腺癌裸鼠模型;用乌骨藤提取物苷晶体(I、II)(0.24 g/250 m L)分别给实验组裸鼠灌胃0.5 m L/只,对照组给饮用水,每日1次,连续30 d。每周测量体质量和肿瘤大小2次,30 d给药后处死小鼠,解剖,称其肿瘤质量,计算抑瘤率,评价乌骨藤提取物苷晶体(Ⅰ、Ⅱ)对裸鼠荷瘤的抑制作用。结果乌骨藤苷晶体Ⅰ和晶体Ⅱ对人宫颈腺癌荷瘤裸鼠有抑制肿瘤作用,抑瘤率分别88.95%和66.71%,其中晶体Ⅰ和Ⅱ抑制肿瘤作用与对照组比较有显著性差异(晶体Ⅰ,P<0.01;晶体Ⅱ,P<0.05)。结论乌骨藤苷晶体Ⅰ和晶体Ⅱ均有抑制人宫颈腺癌荷瘤裸鼠肿瘤的作用。
Objective To study the inhibiting effect of glaucescent fissistigma root crystals in the nude mice with cervical adenocarcinoma; Method Using cervical adenocarcinoma inoculated to the nude mice and growing the solid tumor, subcutaneous transplantation the 3th the solid tumor to establish nude mice model. Take glaucescent fissistigma root crystals （ Ⅰ,Ⅱ） from gastric for experimental, 0.5 mL/one, take water saline as control group , one time per day, 30 days in a row. Measuring the weight and tumor size 2 times in a week, after 30 days of treatment to put to death in mice, anatomize the mice and weighted tumor, calculate the tumor-inhibition rate. Evaluating the tumor-inhibit effection by glaucescent fissistigma root crystal Ⅰ and crystal Ⅱ in nude mice with cervical adenocarcinoma; Result Glaucescent fissistigma root crystal Ⅰ and crystal Ⅱ have affection to nude mice with cervical adenocarcinoma, the anti-tumor rate are 88.95% and 66.71% , respectively. The tumor suppression of crystal Ⅰ have significant difference （P 〈 0.01 ）, crystal Ⅱ （P 〈 0.05） ; Conclusion Glaucescent fissistigma root crystal Ⅰ and crystal Ⅱ can inhibit nude mice with cervical adenocarcinoma to growth.
出处
《实验动物科学》
2014年第6期7-10,共4页
Laboratory Animal Science
基金
江西省卫生厅资助项目(No.2013A021)
关键词
乌骨藤苷
宫颈腺癌
抑制
glaucescent fissistigma root
cervical adenocarcinoma
inhibit
