摘要
目的:研究通关藤苷G在不同pH、温度、光源、金属离子和氧化条件下的降解动力学特征。方法:采用HPLC法测定不同条件下通关藤苷G的含量。根据不超过250 h研究窗口内所得数据,将其含量随时间的变化分别与零级、一级和二级化学反应公式进行拟合,尝试建立降解动力学模型。结果:在pH 2.5溶液中,通关藤苷G的降解最接近二级动力学方程,理论半衰期(t_(1/2))为188.09 h。在35℃、70℃、日光灯和紫外灯条件下,通关藤苷G的降解所得数据与3个动力学方程的区别不大,理论t_(1/2)均大于900 h。在Fe^(3+)溶液中,通关藤苷G的降解最接近一级动力学方程,理论t_(1/2)为38.09 h。在30%H_(2)O_(2)溶液中,通关藤苷G的降解最接近零级动力学方程,理论t_(1/2)为31.25 h。在考察的其他条件下,通关藤苷G较稳定。结论:强酸性溶液、高温环境、日光灯、紫外灯、Fe^(3+)和30%H_(2)O_(2)溶液均会加速通关藤苷G的降解,故其在生产、保存和运输过程中应尽量避免接触上述条件。
OBJECTIVE To study the degradation kinetics of tenacissoside G under different conditions,such as pH,temperature,lights,metal ions,and oxidative environment.METHODS HPLC was used to determine the content of tenacissoside G under the aforementioned conditions during a time span of no longer than 250 h.Based on the data of content vs.time,the degradation behaviors of tenacissoside G were evaluated against kinetic models of zero-,first-,and second-order chemical reactions.RESULTS Under the condition of pH 2.5 solution,degradation of tenacissoside G approximated best to second-order kinetic equation,with the theoretical half-life time(t_(1/2))of 188.09 h.Under the conditions of 35℃,70℃,fluorescent and UV lights,its degradations did not provide enough data within the testing time window to differentiate among the three kinetic equations,and all the theoretical t_(1/2)were longer than 900 h.Under the condition of Fe^(3+)solution,degradation of tenacissoside G approximated best to first-order kinetic equation,withthe theoretical t1/2of 38.09 h.Under the condition of 30%H_(2)O_(2) solution,degradation of tenacissoside G approximated best to zero-order kinetic equation,with the theoretical t_(1/2)of 31.25 h.Tenacissoside G was relatively stable under other conditions examined.CONCLUTION Strong acidic solution,high-temperature,fluorescent and UV lights,Fe^(3+)and 30%H_(2)O_(2) solutions can accelerate tenacissoside G degradation,so it would be recommended to avoid such conditions during its production,storage,and transportation.
作者
孟礼
王莉莉
郭明
MENG Li;WANG Li-li;GUO Ming(School of Pharmacy,Nanjing University of Chinese Medicine,Jiangsu Nanjing 210023,China)
出处
《中国医院药学杂志》
CAS
北大核心
2023年第8期882-886,共5页
Chinese Journal of Hospital Pharmacy
基金
重大新药创制科技重大专项(编号:2018ZX09711002-011-007)。
关键词
通关藤
通关藤苷G
降解动力学
稳定性
HPLC
Marsdenia tenacissima
tenacissoside G
degradation kinetics
stability
HPLC
