摘要
目的:建立通关藤中通关藤苷G和I的含量测定方法,并比较不同产地通关藤中两者的含量。方法:应用HPLC法,采用Ecosil C18(4.6 mm×150 mm,5μm)色谱柱,以乙腈-水(48∶52)为流动相,流速1.0 m L·min-1,检测波长230 nm,柱温30℃。结果:通关藤中通关藤苷G和I分别在0.412 4~4.124 0μg(r=0.999 5)和0.159 6~1.596 0μg(r=0.999 7)范围内线性关系良好,平均回收率分别为99.5%(RSD=2.4%)和100.0%(RSD=2.4%),不同产地药材中通关藤苷G和I含量范围分别为0.472%~0.882%和0.118%~0.362%。结论:本法准确度,重复性好,专属性强,可控制药材的质量;且不同产地药材中通关藤苷G和I 2种成分含量有一定差异性,其中云南丽江含量最高,可指导临床用药。
Objective:To develop an HPLC method for determination of the proportion of tenacissosides G and I in the caulis of Marsdenia tenacissima(Roxb.)Wight et Arn.from different origins,so as to select the best origins of Marsdenia tenacissima(Roxb.)Wight et Arn.Methods:An HPLC method was established using Ecosil C18 column(4.6 mm×150 mm,5 μm).The mobile phase was acetonitrile-water(48∶52),the flow rate was 1.0 m L· min-1,the detection wavelength was 230 nm,and the column temperature was 30 ℃.Results:The calibration curves of tenacissosides G and I were linear in the ranges of 0.4124-4.1240 μg(r=0.999 5)and 0.159 6-1.596 0 μg(r=0.999 7),respectively.The average recoveries of tenacissosides G and I were 99.5%(RSD=2.4%)and 100.0%(RSD=2.4%),respectively.The contents of tenacissosides G and I in the caulis of M.tenacissima from different origins were 0.472%-0.882% and 0.118%-0.362%.Conclusion:The established method,which is accurate,reproducible and precise,can be applied to the quality control of tenacissosides G and I in the caulis of M.tenocissimae. The content of two ingredients in herbs of different origins are different,among which the highest in samples from Lijiang and lowest from Guiyang;these results will benefit the clinical is found in.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2016年第4期607-610,共4页
Chinese Journal of Pharmaceutical Analysis
基金
辽宁省科技厅自然基金(2014010052-301)
沈阳市科技局优秀人才(F14-231-1-18)
国家科技部科研专项(201407002)
关键词
通关藤
通关藤苷G
通关藤苷I
通关藤含量测定
高效液相色谱法
Marsdeniae tenacissimae Caulis
tenacissoside G
tenacissoside I
Marsdenia tenacissima(Roxb.) Wight et Arn
assay
HPLC