摘要
为探讨内源激素在高秆和矮秆芝麻株高建成中的含量变化特征及其与株高生长速率间的关系,以株高差异显著的2个高秆和2个矮秆芝麻为材料,用液相色谱-串联质谱法(LC-MS/MS)测定相同种植条件下不同生育阶段茎尖和倒三叶内3种激素(生长素IAA、赤霉素GA3和脱落酸ABA)含量。分析发现:从3对真叶期到终花期高/矮秆芝麻株高持续增高,但高秆基因型日均生长速率始终高于矮秆基因型;高/矮秆芝麻株高构建的不同发育阶段,叶片IAA和茎尖GA3含量变化具有较明显差异;ABA含量在高/矮秆芝麻间无明显规律,但在矮秆基因型ZZM2748中表现出一个明显特征,即叶片和茎尖中均在初花后20d含量明显高于其它基因型。从3种内源激素与生长速率相关性分析看出,茎尖中GA3含量与株高生长最为密切。
The contents of 3 endogenous phytohormones including indole acetic acid( IAA),gibberellic acid( GA3) and abscisic acid( ABA) in the 3rd leaves from top and stem tips of two dwarfs and two high genotypes were investigated by liquid chromatography- tandem mass spectrometry,in order to analyze their dynamic changes and their relationship to growth rate. The results showed that these 2 genotypes kept growing from the 3- true- leaf stage to terminal flowering stage,but the daily growth rate of the high genotypes was greater than that of the dwarf genotypes. During various developmental stages of plant height in 2 types of material,content variation of IAA in leaf and GA3 in stem tip were observed. There was no clear change pattern of ABA content between 2 types of sesame. Compared with other 3 genotypes,the ABA content in ZZM2748 was significantly higher on 20 th day post anthesis( DPA) both in leaf and stem tip and was similar to others during other growth period,indicating that there might be a mechanism that strongly inhibited stem growth. Correlations analysis showed GA3 and stem growth of sesame had the strongest correlation.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2015年第1期83-89,共7页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31271766)
农业部作物种质资源保护项目[NB2011-2130135-29(1)]
中国农业科学院科技创新工程
关键词
芝麻
株高建成
内源激素
生长速率
Sesame
Plant height development
Phytohormone
Growth rate
