摘要
目的观察变应性鼻炎(allergic rhinitis,AR)大鼠鼻黏膜纤毛超微结构改变及18β-甘草次酸对纤毛病理改变的影响。方法将96只Wistar大鼠随机分为空白组、模型组、氯雷他定组、18β-甘草次酸组,每组24只。采用卵清蛋白致敏法建立大鼠AR模型,造模后各组分别给药干预。于给药2、4、6、10周后比较各组大鼠行为学改变,并采用扫描电镜和透射电镜观察各组鼻黏膜纤毛超微结构改变。结果模型组出现典型AR症状,鼻黏膜纤毛紊乱、倒伏、黏附聚集甚至脱落,纤毛膜破裂,微管减少或消失,并出现密集的短小纤毛,纤毛顶端黏液毯增厚并含中性粒细胞、单核细胞、嗜酸性粒细胞等炎性细胞;在变应原持续作用下,模型组鼻黏膜纤毛随时间延长而呈进行性损害改变。经氯雷他定和18β-甘草次酸干预后,AR症状逐渐减轻,鼻黏膜上皮病理损害逐渐恢复,纤毛整齐密集接近空白组,短小纤毛减少,纤毛顶端增厚覆盖的黏胶层消失。结论在大鼠AR模型中,随着变应原的持续接触,鼻黏膜纤毛超微结构呈进行性损害。18β-甘草次酸可一定程度延缓或逆转鼻黏膜纤毛的病理改变。
Objective To observe the ultrastructure changes of nasal mucosa cilia in rat models of allergic rhinitis （AR）, and the effect of 18β-glycyrrhetinic acid on the pathological changes of nasal mucosa cilia. Methods Totally 96 Wistar rats were randomly divided into 4 groups： normal control group, AR model group, loratadine treatment group and glycyrrhetinic acid treatment group. AR models were established by ovalbumin-induction, and then each group was administered with corresponding treatments. At 2 weeks, 4 weeks, 6 weeks, and 10 weeks after treatment, the behavioral changes were compared between different groups and the ultrastructure changes of nasal mucosa cilia were observed under scanning electron microscope and transmission electron microscope in each group. Results Model group developed typical AR symptoms： the cilia in the top layer of the nasal mucosa were disordered, lodging, matted together and some were even lost; the cilia membrane was broken, and the microtubules of cilia were reduced or disappeared; furthermore, small and abnormal cilia were observed, while mucous blanket on the top of cilia was thickened and contained neutrophils, monocytes, eosinophils and other inflammatory cells; and with persistent allergen exposure, the morphological changes of the nasal mucosa cilia aggravated gradually. While in both glycyrrhetinic acid treatment group and loratadine treatment group, the AR symptoms were relieved gradually, and the morphological damages of nasal mucosa were gradually recovered： the cilia were well arranged with the density similar to that of normal group, and small cilia were reduced and the thickened mucous blanket on the top of cilia disappeared. Conclusion Persistent exposure to allergen can lead to progressive ultrastructure damages to nasal mucosa cilia in rat AR model, and 1813-glycyrrhetinic acid can, to some extent, relieve or even reverse the ultrastructure changes of nasal mucosa cilia.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2015年第1期26-33,共8页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(81160449)
甘肃省卫生厅行业科技计划项目(GSWST2011-06)
甘肃省中医药科学技术研究课题(GZK-2011-22)~~
