摘要
目的:研究全反式维甲酸联合奥沙利铂对结肠癌LoVo细胞株增殖、血管新生能力的影响。方法:培养结肠癌LoVo细胞株,分为空白对照组(不含FBS的培养基处理)、ATRA组(10-5 mol/L全反式维甲酸处理)、OHP组(80μg/mL奥沙利铂处理)、联合处理组(10-5 mol/L全反式维甲酸和80μg/mL奥沙利铂共同处理)。采用MTT法检测细胞增殖能力,real-time PCR检测血管新生因子的mRNA含量,酶联免疫吸附法检测血管新生因子的蛋白含量。结果:(1)细胞增殖能力:联合处理组、ATRA组、OHP组的MTT值均低于空白对照组(P<0.05),且联合处理组的MTT值低于ATRA组、OHP组(P<0.05);(2)血管新生因子:联合处理组、ATRA组、OHP组的血管内皮生长因子(VEGF)A、VEGFB、VEGFC、转化生长因子(TGF)β1、缺氧诱导因子(HIF)-1α的表达量均低于空白对照组,且联合处理组的VEGFA、VEGFB、VEGFC、TGFβ1、HIF-1α的表达量低于ATRA组、OHP组(P<0.05)。结论:全反式维甲酸联合奥沙利铂能够抑制结肠癌LoVo细胞株的增殖,降低血管新生因子的表达。
Objective： To study the effects of all trans retinoic acid combined with oxaliplatin on proliferation and angio- genesis ability of colorectai cancer LoVo cell line. Methods~ Colorectal cancer LoVo cell line were cultured and divided into blank control group（treated with culture medium without FBS）, ATRA group（treated with 10^-5 mol/L all trans retinoic acidt）, OHP group （treated with 80/zg/mL oxaliplatin）, combination group（treated with 10 5 mol/L all trans retinoic acidt and 80 μg/ mL oxaliplatin）. Cell proliferation was detected by MTT method, mRNA content of angiogenesis factor were detected by real- time PCR, protein content of angiogenesis factor were detected by enzyme linked immunosorbent assay. Results： MTT values of combined treatment group, ATRA group, OHP group were lower than those of blank control group; MTT values of ATRAgroup, OHP group were lower than combined treatment group. VEGFA, VEGFB, VEGFC, TGFβ1, HIF-1α expression of combined treatment group, ATRA group, OHP group were lower than those of blank control group; VEGFA, VEGFB, VEGFC, TGFβ1, HIF-1α expression of ATRA group, OHP group were lower than those of combined treatment group. Conclusion： All trans retinoic acid combined with oxaliplatin treatment can inhibit the proliferation of colon cancer cell line LoVo and reduce the expression of angiogenesis factors.
出处
《海南医学院学报》
CAS
2014年第12期1610-1613,共4页
Journal of Hainan Medical University
基金
恩施土家族苗族自治州科技局重点项目(201012)~~
关键词
结肠癌
全反式维甲酸
奥沙利铂
增殖
血管新生
Colon cancer
All trans retinoic acid
Oxaliplatin
Proliferation
Angiogenesis
