摘要
本研究旨在揭示影响绵羊重要经济性状的功能基因的分子遗传特征及其与细毛羊群体的遗传关系,为高效选育绵羊品种经济性状及其种质资源的保护与利用提供分子遗传学依据。试验利用PCR-SSCP、DNA测序和生物信息学对289个细毛羊的KRT26基因进行遗传变异分析及其与细毛羊羊毛细度的关联性分析。结果表明,KRT26基因在该细毛羊群体中存在AA、AB、BB 3种基因型,其基因型频率分别为0.221、0.426和0.353,A、B等位基因频率分别为0.434、0.566,细毛羊群体的多态信息含量为0.371,呈中度多态水平,且处于Hardy-Weinberg非平衡状态(P<0.05)。经过BioEdit软件比对序列和Chromas软件分析测序结果显示,KRT26基因发现5处碱基突变:83bp(G/C)、86bp(T/C)、112bp(C/T)、140bp(G/A)和247bp(C/T),并通过氨基酸序列的比对结果表明,2处发生了氨基酸的替代,即Val/Ile和Asn/Lys。KRT26基因在细毛羊群体中AA基因型个体极显著高于AB和BB基因型(P<0.01)。因此,KRT26基因可能作为羊毛细度性状的一个新的分子标记。
The present study was designed to investigate the traits of economic importance of molecular genetic features of functional genes,to evaluate the genetic relationship in fine-wool sheep,to provide molecular genetic basis for efficient breeding of sheep breeds,and to effeciently conserve and utilize the germplasm resources.PCR-SSCP,DNA sequencing and bioinformatics techniques were used to analyze the association of KRT26 with the wool fineness in 289fine-wool sheep.The results showed that in fine-wool sheep population,there were three genotypes and the genotypic frequencies of AA,AB,BB were 0.221,0.426 and 0.353,respectively.The gene frequencies of allele A and B were 0.434 and 0.566,respectively,which was intermediate polymorphism with the polymorphic information content of 0.371 and was not in the agreement with Hardy-Weinberg equilibrium（P〈0.05）.Applying BioEdit and Chromas software,five novel SNPs of KRT26 gene were genetyped,which were 83bp（G/C）,86bp（T/C）,112bp（C/T）,140bp（G/A）and 247bp（C/T）,respectively.Moreover,two novel SNPs were missense which resultedin change in amino acid（SNP 140G/A lead to Val/Ile and SNP 247C/T lead to Asn/Lys）.The association of SNPs with wool fineness trait showed that the wool fineness with AA genotype were extremely significant higher than that with AB and BB genotypes（P〈0.01）.The results implied that the KRT26 gene might be one of important candidate molecular markers associated with the wool fineness trait.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第1期161-166,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家自然基金项目(31360543)
现代农业产业技术体系建设专项:国家绒毛用羊产业技术体系(CARS-40)
