摘要
采用PCR-SSCP的分子标记技术,选择编码羊毛纤维组成蛋白基因中的KAP1.1、KAP1.3的部分序列、KAP6.1的外显子区作为候选基因,通过对其多态性的研究,探索将该基因作为候选基因来间接选择羊毛细度性状的可行性。得出其中在角蛋白辅助蛋白的多基因家族中的高硫蛋白辅助蛋白基因(KAP1.1、KAP1.3)中,位点W08667与羊毛细度有显著的相关性(P<0.05)。在甘氨酸酪氨酸角蛋白辅助蛋白中,外显子位点W06933的AA基因型和BB基因型与羊毛细度之间有显著的相关性(P<0.05)。
Partial sequences of wool fiber constituent genes KAP1.1 and KAP1.3 and the exonic sequence of the KAP6.1 gene were chosen for polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） analysis to assess their ability as candidate genes during indirect selection for fine wool traits. Results show that locus W08667 in the genes （KAP1.1, KAP1.3） which code the high sulfur protein associated-protein of keratin associated-protein family is significantly correlated with fine wool quality （P〈0.05）. Among the high-glycine-tyrosine keratin associated- protein, the AA and BB genotypes of W06933 are also significantly correlated with fine wool quality （P〈0.05）.
出处
《遗传》
CAS
CSCD
北大核心
2007年第1期70-74,共5页
Hereditas（Beijing)
基金
国家"十五"科技攻关项目(编号:2002BA514A-3-2
2002BA514A-8)
国家自然科学基金项目(编号:39869002)
新疆维吾尔自治区高新技术项目(编号:200411104)资助~~
