摘要
目的：建立超高效液相色谱（ UPLC）法测定血清中苯妥英钠、苯巴比妥和卡马西平的浓度。方法血清样品经乙腈沉淀蛋白后进样分析。色谱柱为ACQUITY UPLC BEH C18色谱柱，流动相为乙腈－水（28∶72），流速：0.3 mL· min－1，检测波长220 nm，柱温20℃。结果苯妥英钠、苯巴比妥和卡马西平的血清浓度分别在2.5～80.0，5.0～160.0，1.0～32.0μg· mL－1线性关系良好，日内、日间精密度（ RSD）均小于7.28％，提取回收率为96.18％～103.89％。结论本方法简便、快速、准确，可用于测定血清中苯妥英钠、苯巴比妥和卡马西平的浓度。
Objective To establish an ultra performance liquid chroma-tography （ UPLC） method for the determination of phenytoin sodium , phe-nobarbital and carbamazepine in human serum.Methods Serum sam-ples were injected and analyzed after protein precipitation with acetoni-trile.The analysis was performed on an ACQUITY UPLC BEH C 18 column, and the mobile phase consisted of acetonitrile -water （28∶72）, the flow rate was 0.3 mL · min-1 , the detection wavelength was set at 220 nm and the temperature was maintained at 20 ℃.Results The peak area and content of phenytoin sodium , phenobarbital and carbama-zepine showed good linearity in the range of 2.5-80.0, 5.0-160.0 and 1.0-32.0 μg · mL-1 , respectively.Intra-and inter -day RSDs were all lower than 7.28%. The recoveries of phenytoin sodium were 96.18%-103.89%.Conclusion The established method is simple , accurate and rapid , and can be applied to determination of phenytoin sodium , phenobarbital and carbamazepine in human serum.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第2期119-121,共3页
The Chinese Journal of Clinical Pharmacology
基金
常州四药临床药学科研基金资助项目(CZSYJJ13018)
关键词
苯妥英钠
苯巴比妥
卡马西平
超高效液相色谱法
血清
phenytoin sodium phenobarbital carbamazepine ultra performance liquid chromatography serum
