摘要
目的探讨超高效液相色谱法同时测定三黄片中黄芩苷、盐酸小檗碱、大黄素、大黄酚含量的可行性。方法采用超高效液相色谱法测定三黄片中4种组分的含量。结果黄芩苷在6.03-241.2 mg/L、盐酸小檗碱在6.21-248.4 mg/L、大黄素在2.06-82.4 mg/L、大黄酚在1.93-77.4 mg/L范围内线性关系良好（r≥0.99）;稳定性试验的相对标准偏差（RSD）均小于1.0%（n=6）,精密度试验的RSD均小于1.0%（n=6）,重复性试验的RSD均小于1.5%（n=6）;黄芩苷、盐酸小檗碱、大黄素、大黄酚的平均回收率分别为97.8%、97.0%、99.8%、98.9%,RSD均小于1.5%（n=6）。结论超高效液相色谱法快速、灵敏,结果准确和重现性好,适用于三黄片的质量控制。
Objective To explore ultra performance liquid chromatographic method for the simultaneous determination of the feasibility of Sanhuang tablets;baicalin,berberine hydrochloride,emodin,chrysophanol content. Methods The contents of 4 components determination of sanhuangpian ultra high performance liquid chromatography. Results baicalin in 6.03-241.2mg/L range and berberine hydrochloride in 6.21-248.4mg/L range and emodin in 2.06-82.4mg/L range and Chrysophanol in 1.93-77.4mg/L linearity（r greater than or equal to 0.99） good.The RSD of stability test were less than 1.0%（n=6）and the RSD of precision test were less than 1.0%（n=6） and the RSD of the repeatability of the method were less than 1.5%（n=6）,baicalin,berberine hydrochloride,emodin,chrysophanol,average recovery rate was 97.8%,97.0%,99.8%,98.9% and RSD was less than 1.5%（n=6）.Conclusion Ultra high performance liquid chromatography method is rapid,sensitive,accurate and reproducible,and suitable for the quality control of Sanhuang tablet.
出处
《中国药物经济学》
2016年第5期11-13,共3页
China Journal of Pharmaceutical Economics
