摘要
目的:考察黄芪葛根汤总黄酮、总皂苷对糖尿病大鼠血糖、血脂及肝脏白介素12(IL-12)、白介素15(IL-15)的影响及其在调节糖尿病糖脂代谢过程中的交互作用。方法:将66只大鼠随机分为6组:正常组、模型组(A1B1)、对照组、总黄酮组(A2B1)、总皂苷组(A1B2)、总黄酮和皂苷组(A2B2),每组11只。除正常组外,其余各组用链脲佐菌素(STZ)造模后,按2×2析因设计实验方案实施实验,分别于造模前、造模后第7天测定大鼠随机血糖,造模后30天测定血糖、血脂以及肝脏IL-12、IL-15等指标。有关联的指标计算加权综合评分,对综合指标运用析因设计方差分析方法分析总黄酮、总皂苷的主效应及其交互效应。结果:与正常组比较,模型组各项指标差异均有统计学意义(P<0.05)。总黄酮、总皂苷均可降低大鼠血糖水平,两者之间无交互作用;总黄酮、总皂苷均能降低血清胆固醇(CHO)、甘油三酯(TG)、IL-15、IL-12水平,两药之间有交互作用,单一用药比合用效果好。结论 :黄芪葛根汤总黄酮、总皂苷具有降低糖尿病大鼠血糖、CHO、TG水平以及肝脏IL-12、IL-15水平的作用;但总黄酮、总皂苷对上述指标的调节未呈现叠加协同作用。
This study was aimed to explore the effect of total flavonoids and saponins from Huang-Qi Ge-Gen （HQGG） decoction on blood glucose （BG）, serum lipid, interleukin-12 （IL-12） and interleukin-15 （IL-15） of liver in diabetes mellitus （DM） rats, in order to investigate their interactions in regulating DM processes. A total of 66 SD rats were randomly divided into 6 groups, which were the normal group, model group （A1B1）, control group, total flavonoids group （A2B1）, total saponins group （A1B2）, and total flavonoids and saponins group （A2B2）, with 11 rats in each group. Except the normal group, other groups were intraperitoneal injected with streptozotocin （STZ）. And the experiment was according to 2×2 factorial design experiment scheme. The BG was determined before STZ injection and 7 days after the STZ injection. After 30 days, BG, serum lipid, IL-12 and IL-15 of liver were tested. Related indexes were calculated to the weighted composite score. Main and interactive effect of total flavonoids and saponins were studied according to the factorial design experiment scheme. The results showed that compared with the normal group, all indexes of model group showed statistical differences （P〈0.05）. Total flavonoids and saponins from HQGG decoction can effectively reduce BG, without any interactions between them. Both the total flavonoids and total saponins can reduce serum cholesterol （CHO）, triglyceride （TG）, liver IL-12 and IL-15. And there were interactive effects. The single use of herb achieved better effects than the combination. It was concluded that total flavonoids and saponins from HQGG decoction can reduce BG, CHO, TG, and liver IL-12 and IL-15 levels in rats. However, the regulation of total flavonoids and saponins on indexes mentioned above showed no additive effect.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第11期2475-2479,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委重大课题(81273653):黄芪有效部位选择性发挥效应的规律与功能机制研究
负责人:范颖
