摘要
目的观察生芪降糖颗粒干预胰岛素抵抗(IR)模型大鼠的作用,并探讨其作用机制。方法选取Wistar大鼠以高脂饮食、链脲佐菌素腹腔注射制造IR模型,随机分组分别给予不同剂量的生芪降糖颗粒、罗格列酮治疗,同时设立正常对照组,观察血糖以及主动脉内皮素-1(ET-1)、蛋白激酶(PKC)蛋白表达的变化。结果生芪降糖颗粒中、高剂量组和罗格列酮组大鼠的血糖均明显下降,并以生芪降糖颗粒高剂量组下降最明显。模型组主动脉ET-1、PKC表达量较正常组显著升高(P<0·05)。罗格列酮组和生芪降糖颗粒高剂量组主动脉ET-1、PKC表达量较模型组均下降,与模型组比较差异具有显著性(P<0·05),且呈剂量依赖性。结论生芪降糖颗粒通过降糖、抑制ET-1、PKC表达等作用,改善血管内皮功能,从而起到胰岛素增敏及减轻胰岛素抵抗的作用。
Objective To study the effect of Shengqi Jiangtang Granule （SJG） on tance （IR） and explore the mechanism. Methods IR model was induced by high fat rat model of insulin resis diet and peritoneal injec tion of streptozotocin in Wistar rats. Model rats were randomly divided into the model group, the low, medium and high dose SJG groups treated with corresponding dose of SJG, and the RGL group with rosiglitazone （RGL）. A normal control group was also set up. Blood glucose （BG）, protein expression of endothelin-1 （ET- 1） and protein kinase C （PKC） in aortic tissue were detected. Results BG decreased in the high and medium dose SJG groups and the RGL group after treatment, and the lowest level was shown in the high dose SJG group. Compared with that in the normal group, the expressions of ET-1 and PKC obviously increased in the model group （P〈0.05）, which was lowered in the RGL group and the high and medium dose SJG groups after treatment in a dosage-depending manner, showing significant difference to those in the model group （P 〈 0.05）. Conclusion SJG can lower blood glucose and inhibit ET-1 and PKC expressions to improve vascular endothelial function, thus to promote insulin sensitiveness and alleviate IR.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2007年第5期449-451,共3页
Chinese Journal of Integrated Traditional and Western Medicine
基金
博士之窗2004年青岛市科技局课题(No.KZD-04)
