摘要
目的：观察益精方对环磷酰胺所致少弱精子症模型小鼠精子线粒体功能的影响。方法将40只雄性昆明小鼠随机分为对照组（CG）、模型组（MG）、小剂量中药治疗组（SG）和大剂量中药治疗组（LG）。按60mg/kg体质量给CG小鼠腹腔注射生理盐水（NS），给MG、SG、LG小鼠腹腔注射环磷酰胺，每天1次，连续5d，第6天开始按体质量分别给SG和LG小鼠灌服益精方，剂量分别为人类常规用量（以60 kg为标准）的1倍和5倍，MG小鼠给予等体积的NS灌胃，每天1次，连续34 d，CG常规饲养，然后对小鼠进行精液常规分析，流式细胞术检测精子线粒体功能。结果 CG、MG、SG和LG 组小鼠附睾精子密度分别为（5.20±1.34）、（1.73±0.03）、（2.08±0.01）、（3.31±0.56）×10^6/mL；精子活力[（a＋b）级精子百分率]分别为（14.49±0.30）%、（6.64±1.88）%、（11.99±1.01）%、（19.40±3.13）%；精子活率[（a＋b＋c）级精子百分率]分别为（68.39±15.13）%、（39.96±4.89）%、（62.28±4.43）%、（73.61±5.05）%。MG组精子密度、精子活力、精子活率显著低于CG组（P＜0.05）；经治疗后LG组精子密度、精子活力和精子活率明显增加，与MG组比较有显著性差异（P ＜0.05），而与CG组在精子活力和精子活率上没有明显区别。在CG、MG、SG和LG组，线粒体功能良好精子的百分率分别为（1105.93±0.33）%、（848.55±6.15）%、（888.77±22.46）%、（1000.885±72）%，线粒体功能丧失精子的百分率分别为（27.94±0.32）%、（42.43±1.00）%、（35.11±1.23）%、（30.91±0.44）%，MG小鼠精子线粒体功能良好的表达量明显下降，而线粒体功能丧失的表达量明显增多，与CG小鼠比较均有明显差异（P＜0.05）；经治疗后， LG小鼠精子线粒体功能良好的表达量显著性增加（P＜0.05），同时线粒体功能丧失的表达量明显减少，与MG小鼠比较有明显差异（P ＜0.05�
Objective To observe the effects of Yijingfang on the mitochondrion in the mouse model of cyclophosphamide-induced oligoasthenospermia. Methods Forty Kunming male mice were randomly divided into four groups such as the control group （CG）, the model group （MG）, small-doseYijingfang group （SG）, and large-doseYijingfang group of （LG）. The mice of CG were intraperitoneally injected with normal saline at 60 mg/kg once a day ,while those of MG, SG and LG with cyclophosphamide, all for 5 days. During the next 34 days, the mice of SG and LG received intragastric administration ofYijingfang once a day, the former at a dose 2times and the latter 5 times that of human routine usage, those of MG given the same volume of normal saline, and CG normally fed. At 35 days, we measured the sperm count, percentages of grades a＋b and a＋b＋c sperm, and the function of mitochondrion in the epididymal sperm of the mice. Results The sperm counts of CG, MG, SG and LG were（5.20±1.34）, （1.73±0.03）, （2.08±0.01） and （3.31±0.56）×10^6/mL, respectively, and sperm number was significantly lower in MG than that in CG （P〈0.05）, but higher in LG than that in MG （P〈0.05）. The grade a＋b sperm constituted （14.49±0.30）, （6.64±1.88）, （11.99±1.01） and （19.40±3.13）% in CG, MG, SG and LG, respectively, and the grade a＋b sperm was remarkably lower in MG than that in CG （P〈0.05）, but higher in LG than that in MG（P〈0.05）; the grade a＋b sperm accounted for （68.39±15.13）, （39.96±4.89）, （62.28±4.43） and （73.61±5.05）%, respectively, and it was obviously lower in MG than that in CG （P〈0.05） but higher in LG than that in MG （P〈0.05）; the percentages of good function mitochondrion were （1105.93±0.33）%, （848.55±6.15）%, （888.77±22.46）% and （1000.885 ±72）% in CG, MG, SG and LG, respectively, and it was remarkably lower in MG than that in CG （P〈0.05）, but higher in LG than that in MG（P〈0.05）; defuctionalization mitoch
出处
《中国男科学杂志》
CAS
CSCD
北大核心
2014年第10期10-14,共5页
Chinese Journal of Andrology
关键词
益精方
线粒体
弱精子症
小鼠
Yijingfang
mitochondria
asthenozoospermia
mice
