摘要
研究利用特异引物对2份抗病纯合材料(基因型Mi/Mi)和2份感病纯合材料(基因型mi/mi)进行PCR扩增,抗、感材料均产生750 bp的PCR扩增片段,纯合抗病和杂合抗病材料的PCR产物存在Taq I酶切位点,酶切后分别产生了570 bp和180 bp以及750 bp、570 bp和180 bp的片段,而感病材料的扩增产物无此酶切位点,酶切后仍为750 bp的片段。利用该标记对4份番茄杂交种进行检测,其中2份杂交种表现为杂合抗病型,另2份杂交种表现为纯合感病型。利用该标记对其中一个表现为杂合抗病的杂交种50份F2代单株进行检测,抗感遗传的分离比符合3∶1。说明该分子标记的这一抗病基因属于单基因控制的质量性状遗传位点。
In this paper,two resistant homozygous lines （Mi /Mi ）and two homozygous susceptible lines （Mi /Mi ）were amplified by specific primer.Both resistant and susceptible lines produced about 750 bp PCR frag-ment .After digestion with enzyme Taq I ,homozygous resistant genotypes and heterozygous resiatant genotypes could produce fragment about 570 bp,180 bp and 750 bp,570 bp,180 bp,respectively.While the susceptible genotypes still presented 750 bp fragment.Four hybrids were detected using this marker,and the results show that two hybrids are resistant heterozygous and two hybrids are susceptible.The marker also were used to de-tect F2 generation of 50 plants,the Separate ratio of resistant plants and susceptible plants is 3∶1 ,so genetic traits should be controlled by the quality characters.
出处
《辽宁农业科学》
2014年第6期11-14,共4页
Liaoning Agricultural Sciences
基金
国家科技支撑计划项目(2012BAD02B02-5)
关键词
番茄根结线虫病
MI
分子标记
Tomato root-knot nematode Disease Mi Molecular marker
