摘要
会阴花纹是根结线虫种类鉴定的主要依据,它的纹路变化对于判断根结线虫的种类具有重要意义。由于根结线虫的个体小,在进行会阴花纹的制片和显微观察时,常常遇到制作易败,观察模糊的现象,因而种类的正确鉴定受到较大影响。在会阴花纹的常规制片基础上,从根结线虫成虫的获得、会阴花纹的切取、片子的制作等方面进行了一定的改进,主要是用甲烯蓝对其会阴花纹进行了处理,其结果与未处理的相比,具有制作简单、快捷,显微观察纹路清晰、图案易辩、利于判断等优点,对于根结线虫的种类鉴定有一定的应用价值。
Perineal-striae are very important for the species identification of root-knot nematodes. But the root-knot nematodes are very small. And it was difficult to make slices about vulva striae of root-knot nematodes now. In this article, we improved the traditional methods in slicing,cutting,dyeing. Especially, the new last step-dyeing with Methylthionine Chloride was very important for quality of Slices. After the dyeing, perineal-striae became clearer. At the same time, it was conducive to get a better result.
出处
《北方园艺》
CAS
北大核心
2008年第3期207-208,共2页
Northern Horticulture
关键词
鉴定
会阴花纹
染色
Identification
Perineal-striae
Dyeing
