摘要
槲皮素是灯盏花中的有效黄酮化合物之一,为此对灯盏花中槲皮素的提取方法进行改进.采用真空超声法提取,联用高效液相色谱进行测定,最优提取条件为,提取溶剂为40%的乙醇溶液,提取时间为15 min,固液比为1∶20.测定结果为,真空超声提取法的萃取量为0.093 5 mg/g,优于常压超声提取法的萃取量0.042 3 mg/g,改进后的真空超声提取法有利于溶质的溶出、避免了黄酮化合物的氧化.
Quercetin is one of the effective flavonoids in Erigeron Breviscapus.The extraction method of quercetin in Erigeron Brevisca-pus was improved by using ultrasonic extraction under vacuum.Quercetion was determined by high performance liquid chromatography. The optimized conditions of extraction solvent,extraction time and solid-liquid ratio were 40%ethanol,15 min and 1∶20,respectively. The results show that the extraction amount was 0.093 5 mg/g for ultrasonic extraction under vacuum while 0.042 3 mg/g for conven-tional ultrasonic extraction,which showed the better extraction efficiency.The improved method was benefit to the dissolution of solutes and avoided oxidation of flavone compounds.
出处
《昆明学院学报》
2014年第6期39-41,共3页
Journal of Kunming University
基金
国家自然科学基金资助项目(21205054)
云南省应用基础研究资助项目(2011FZ043)
昆明理工大学分析测试基金资助项目(20140956
20140597)
大学生科技创新资助项目(20131125111020
20131125111021)
关键词
槲皮素
灯盏花
真空超声提取法
高效液相色谱法
quercetin Erigeron Breviscapus ultrasonic extraction under vacuum high performance liquid chromatography
