摘要
研究了鲫鱼在肝胰脏抗氧化酶活性、肝胰脏铜含量和血液红细胞微核等不同层面对铜胁迫的响应和甘草的解毒功能。试验分3组,自来水对照组、水中加硫酸铜组、加铜和甘草组,连续处理7d。结果表明,处理2、3、4d时,加铜组中鲫鱼肝脏超氧化歧化酶、过氧化物酶活性及丙二醛含量均显著高于自来水对照组,随时间延长超氧化歧化酶、过氧化物酶活性逐渐升高,而丙二醛含量却逐渐下降。加入甘草后,鲫鱼肝脏超氧化歧化酶、过氧化物酶活性及丙二醛含量均低于加铜组。处理4、7d时加铜和甘草组中鲫鱼肝脏铜含量分别为13.08mg/kg、14.96mg/kg,高于加铜组的10.83mg/kg、11.64mg/kg。处理7d时,加铜和甘草组中鲫鱼外周血红细胞微核率为3.17%低于加铜组的4.17%,这说明甘草具有一定的解毒功能。
Crucian carp （Carassius auratus） was bathed in tapwater （control group） ,water containing cop‐per and water supplemented with licorice for 7 days to evaluate detoxification function of licorice and re‐sponse of crucian carp to Cu2＋ stress w ere studied via antioxidant enzyme activity ,Cu2＋ content and micro‐nucleus levels in red cells . Results showed that there were significantly higher superoxide dismutase （SOD） ,and peroxidase （POD） activities and malonaldehyde （MDA） content in the fish bathed in the wa‐ter containing copper than those in the control group when the fish were bathed in the Cu2＋ containing wa‐ter for 2 ,3 and 4 days .The activities of SOD and POD were increased significantly as Cu2＋ exposure elasped ,but MDA content gradually decreased .The fish bathed in the licorice containing water had lower SOD ,and POD activities and MDA content in hepatopancreas than the fish in the control group .There was Cu2＋ content of 13 .08 mg/kg and 14 .96 mg/kg in hepatopancreas of the fish bathed in the Cu2＋ con‐taining water for 4 and 7 days ,significantly higher than those in the fish bathed in the licorice containing water （10 .83 mg/kg and 11 .64 mg/kg） .There was significantly lower micronucleus rate in the fish bathed in the licorice containing water（3 .17% ） than that in the fish bathed in the Cu2＋ containing water （4 .17% ） for 7days ,indicating that licorice has a certain detoxification function .
出处
《水产科学》
CAS
北大核心
2014年第11期713-717,共5页
Fisheries Science
基金
新疆高校科研计划项目(XJEDU2011S39
XJEDU2012S37)
关键词
甘草
抗氧化酶
微核
Carassius auratus
antioxidant enzyme
micronucleus
