摘要
通过微核和彗星试验检测了铜离子胁迫下锦鲤(Cyprinus carpio)外周血细胞DNA损伤情况。实验发现不同浓度铜离子胁迫48 h后,锦鲤外周血细胞的彗星率(17. 7%,44. 4%,60. 0%,88. 9%)和微核率(0. 4‰,8. 4‰,9. 8‰,12. 2‰)与铜离子浓度呈正相关。细胞尾部DNA含量(2. 91%,5. 74%,8. 43%,11. 28%)、尾长(47,74,108,112μm)和Olive尾距(4. 51,9. 39,17. 68,18. 62)也都随铜离子浓度升高而呈增长趋势,而且G2,G3组均显著高于对照组(P <0. 05)。2种试验方法所获结果相互印证,从不同角度揭示了铜离子对锦鲤外周血细胞的遗传毒性效应。特别是微核试验灵敏度较高,步骤简捷,成本低廉,在生态遗传毒性检测应用推广方面具有较大优势。
Common carp(Cyprinus carpio)was cultured and exposed to different concentrations of Cu^2+,and the DNA damage of the peripheral blood cells of C.carpio was analyzed by using the micronucleus test and the comet assay.The results showed that the rates of comet(17.7%,44.4%,60.0%,88.9%)and micronucleus(0.4‰,8.4‰,9.8‰,12.2‰)of the peripheral blood cells of C.carpio in the control,G1,G2 and G3 were positively correlated with the concentration of Cu^2+after C.carpio were exposed to Cu^2+for 48 hrs.Under the stress of Cu^2+for 48h the tail DNA content(2.91%,5.74%,8.43%,11.28%),tail length(47,74,108,112μm)and Olive tail moment(4.51,9.39,17.68,18.62)in the control,G1,G2 and G3 all tended to increase with the rise of Cu^2+concentration and they were significantly higher in the groups G2 and G3 than in the control group(P<0.05).The results of either the micronucleus test or the comet assay corroborated the genetic damage by Cu^2+to the peripheral blood cells of C.carpio from different aspects.In particular,the micronucleus test was high in sensitivity,low in cost,and simple in steps and will have a high potential of application and extension in the test of ecogenotoxicity.
作者
刘秋妤
陈新
覃宗敏
卓文
唐敏
LIU Qiuyu;CHEN Xin;QIN Zongmin;ZHUO Wen;TANG Min(College of Materials and Chemical Engineering,Hainan University,Haikou,Hainan 570228,China;State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University),Haikou,Hainan 570228,China)
出处
《热带生物学报》
2018年第4期388-392,共5页
Journal of Tropical Biology
基金
国家自然科学基金项目(31360105
31660128)
海南省研究生创新科研课题(Hys2018-68
Hys2018-67)
关键词
锦鲤
铜胁迫
DNA损伤
Cyprinus carpio
Cu^2+stress
DNA damage
