摘要
目的 从能量生成角度研究京尼平（Genipin）对激素非依赖性前列腺癌（AIPC）的抑制作用是否与线粒体解耦联蛋白2（UCP2）被阻断有关,并探讨其临床意义.方法 以低、中、高（40、80、120 μmol/L）剂量Genipin干预PC-3细胞培养基48 h,噻唑蓝（MTT）和流式细胞仪检测细胞增殖及凋亡,实时荧光定量聚合酶链反应（Real-time PCR）、Western blot检测UCP2表达,荧光探针检测细胞内Ca2含量,分光光度计检测线粒体膜电势（△ψm）、膜通透性转换孔（mPTP）变化及三磷酸腺苷（ATP）含量.结果 Genipin能显著抑制PC-3细胞增殖,其中对照组及低、中、高剂量组细胞凋亡率分别为（7.60±1.48）％、（9.38±1.01）％、（20.77±1.14）％、（23.48±1.99）％,Genipin可降低UCP2表达,此时细胞内Ca2含量明显增加,且mPTP开放程度增加及△ψm减小,ATP生成明显减少,其中对照组及低、中、高剂量组ATP含量分别为（0.120 ±0.015）、（0.110 ±0.013）、（0.080 ±0.007）、（0.050 ±0.008） mmol/L,以上改变均与Genipin浓度呈正相关,且各组间比较差异均有统计学意义（P＜0.05）.结论 Genipin能明显抑制AIPC细胞的增殖,该抑制作用可能与其阻断UCP2,导致线粒体功能异常,ATP生成减少有关.
Objective To investigate whether uncoupling protein 2 （UCP2） blocked was participated in genipin inhibiting proliferation of androgen independent prostate cancer cells from the perspective of energy,and to explore its clinical significance.Methods PC-3 cells were cultured in vitro,three concentrations of genipin solutions were added to the culture medium.The differences in proliferation,apoptosis,expressions of UCP2,levels of Ca2＋,mitochondrion membrane potential （△ψm）,membrane permeability transition pore （mPTP） and adenosine triphosphate （ATP） between before and after treated with various concentrations of genipin were measured by methyl thiazolyl tetrazolium （MTT） assay,flow cytometry assay,Real-time polymerase chain reaction （PCR） assay,Western blotting assay,spectrophotometer assay respectively.Results Genipin significantly suppressed the growth of PC-3 cells.After treatment with different concentration of genipin for 48 h,the apoptosis rate of PC-3 cells was （7.60 ± 1.48） %,（9.38 ±1.01） %,（20.77 ± 1.14） % and （23.48 ± 1.99） % respectively,expression of UCP2 was significantly reduced,contents of Ca2＋ and mPTP open degree were significantly increased,level of △ψm and contents of ATP were significantly reduced simultaneously,the contents of ATP was （0.120 ± 0.015）,（0.110 ± 0.013）,（0.080 ± 0.007） and （0.050 ± 0.008） mmol/L respectively.The above changes with genipin as a dose-dependent manner,and the differences were statistically significant （P 〈 0.05）.Conclusion Genipin significantly inhibited the growth of androgen independent prostate cancer cells,of which may occur through down-regulating the expression of UCP2,resulting in mitochondrial dysfunction and ATP production decreased.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第12期2664-2666,共3页
Chinese Journal of Experimental Surgery
基金
贵阳市科技局资助项目（筑科合同[2011103]26号）
关键词
激素非依赖性前列腺癌
京尼平
解耦联蛋白2
抑制
Androgen independent prostate cancer cells
Genipin
Uncoupling protein 2
Inhibition
