摘要
目的:探讨京尼平(Genipin)对肾癌是否有治疗作用,该治疗作用是否与线粒体解偶联蛋白2(UCP2)相关。方法:将0、40、80和120μmol/L 4种浓度的Genipin溶液分别浸染肾癌细胞48 h,MTT法和流式细胞术检测细胞的增殖和凋亡情况;聚合酶链反应和ELISA方法检测UCP2的基因和蛋白表达;使用荧光探针检测细胞内钙离子和活性氧分子(ROS)含量。结果:Genipin在中、高剂量组能明显抑制肾癌细胞的增殖并促进细胞的凋亡,随着给药浓度的增加,细胞增值抑制作用及细胞凋亡率逐渐增高(P<0.005)。Genipin能抑制肾癌细胞中UCP2的表达,在中、高剂量给药组,UCP2的基因及蛋白的表达显著抑制(P<0.05)。Genipin能提高细胞内钙离子和ROS的含量,在中、高剂量给药组出现了显著提高(P<0.05)。结论:Genipin能明显抑制肾癌细胞的增殖,促进其凋亡,UCP2可能参与其作用机制。
Objective：To investigate the therapeutic effect of genipin on renal cell carcinoma,and the relationship of UCP2. Methods：Renal carcinoma cells were treated with different concentrations （0,40,80,120 μmoL/L）of genipin. Cell growth inhibition and apoptosis were detected by assay of MTT and flow cytometry. Expression of UCP2 was detected by PCR and ELISA. Contents of ROS and Ca2＋ were tested by fluorescence probe. Results：Genipin significantly suppressed the growth and promoted apoptosis rate of renal carcinoma cells,statistical significance were found in 80 and 120 μmol/L groups. Expression of UCP2 was significantly increased in 80 and 120 μmol/L groups. Genipin improved contents of ROS and Ca2＋ ,and statistical significance were found in 80 and 120 μmol/L groups also. Conclusion： Genipin can suppress renal carcinoma cells growth, and UCP2 may be involved in its mechanism.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期444-448,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
贵州省科技厅社会攻关计划项目(黔科合sy字[2011]3060)
贵阳市科技计划项目(筑科合同[2011103]26号)
