摘要
分别采用L9(34)和L16(45)正交试验方法,研究了观赏羽扇豆组织培养过程中污染和玻璃化控制的最佳组合,探讨吸附剂(活性炭、聚乙烯吡咯烷酮)和抗氧化剂(维生素C、柠檬酸)对外植体褐化的影响。结果表明:0.1%升汞是影响外植体消毒的主要因素,0.1%高锰酸钾次之;采用0.1%高锰酸钾、70%乙醇和0.1%升汞依次消毒30 min、40 s和9 min效果较好,污染率最低为14.8%。培养基中添加活性炭2.0 g/L,培养周期控制在25 d之内,平均褐化率可控制在5.3%,效果最理想;培养基中添加柠檬酸5.0 g/L也能有效控制褐化(8.5%),但整体效果不如活性炭。与正常苗相比,玻璃化苗组织含水量和可溶性糖含量显著升高,叶绿素a、叶绿素b、叶绿素总量、干物质积累和淀粉含量显著降低;温度是影响玻璃化的关键因素,培养基中添加蔗糖30 g/L和琼脂6 g/L,在20℃培养条件下,光照14 h,玻璃化率最低为3.1%。
The optimal combination to control pollution and hyperhydricity in tissue culture of ornamental lupine was studied by L9（34）and L16（45）orthogonal experiments,and the prevention of browning by applica-tion of absorbents （ active carbon,polyvinylpyrrolidone） was probed and antioxidants （ Vitamin C,citric acid） was probed.The result showed that 0.1%HgCl2 was the key factors for explants sterilization,followed by 0.1%K2 MO4 .The lowest pollution rate was 14.8%when sterilized successively with 0.1%K2 MO4 ,70%ethanol and 0.1%HgCl2 for 30 min,40 s and 9 min.When 2.0 g/L active carbon was added to the culture medium and the culture cycle was controlled in 25 days,the browning rate could be effectively reduced to 5.3%,which was the best treatment in this study.The browning was also controlled by adding 5.0 g/L citric acid.However,the effect of citric acid on browning control was not as good as that of active carbon.Compared to the normal shoots,the water and soluble sugar contents were significantly increased,while the contents of chlorophyll a,chlorophyll b, total of chlorophyll,dry matter accumulation and starch were decreased significantly.In addition,temperature was the main factor to cause hyperhydric shoots.The lowest hyperhydric rate was 3.1%when 30 g/L sucrose and 6.0 g/L agar were supplemented in the medium under the conditions of 20℃and light 14 h.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2014年第5期1074-1080,共7页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省科技支撑计划项目(20122BBF60111)
江西省科学院"省部共建国家重点实验室培育基地"计划项目(赣科院字[2013]19号-04)
江西省科学院产学研合作资金项目(2013-09)
关键词
羽扇豆
组织培养
污染
褐化
玻璃化
lupine tissue culture pollution browning hyperhydricity
