摘要
目的 探讨缝隙连接在蛛网膜下腔出血后脑血管痉挛病理机制中的作用.方法 120只SD大鼠随机分对照组、假手术组、手术组及治疗组（n=30）,且各组分第1、3、5、7、14天共5亚组（n=6） 视交叉前池二次注血法建立SD大鼠蛛网膜下腔出血动物模型,HE染色鉴定模型,压力机动仪测量基底动脉外径,Western blot检测基底动脉缝隙连接蛋白.结果 手术组较对照组及假手术组变化显著（P＜0.01）,手术组基底动脉外径、Cx 40减低,Cx 43、Cx 45升高,手术组组内不同时间变化显著（P＜0.01）,基底动脉第1天开始痉挛,第7天达峰值,第14 d逐渐缓解;治疗组较手术组变化显著（P＜0.01）,治疗组基底动脉外径、Cx40升高,Cx43、Cx45减低,治疗组组内无显著变化（P＞0.05）,18β-甘草次酸明显抑制脑血管痉挛;对照组、假手术组及治疗组间无显著变化（P ＞0.05）;Cx37未检测到;Cx40与基底动脉呈正相关（P＜0.01,r=0.823）,Cx43与 Cx45呈正相关（P＜0.01,r=0.939）,Cx40与 Cx43、Cx45呈负相关（P＜0.01,r=0.840;P＜0.01,r=-0.839）,基底动脉直径与Cx43、Cx45呈负相关（P＜0.01,r=0.828;P＜0.01,r=-0.823）.结论 Cx43及Cx45在蛛网膜下腔出血后脑血管痉挛的发生中 可能起促进作用,而Cx40则相反;蛛网膜下腔出血后,Cx43与Cx45间存在正相关,Cx40分别与 Cx43及Cx45 间存在负相关;18β-甘草次酸可缓解蛛网膜下腔出血后脑血管痉挛.
Objective To investigate the role of gap junction in the pathomechanism and treatment of cerebral vasospasm after experimental subarachnoid hemorrhage.Methods HE dyed was used to identify the model.The diameters of basilar arteries were determined by pressure myograph.The connexins in the basilar arteries of different groups were examined by western blot.Results The diameters,connexins in basilar arteries were compared in and between groups respectively.There are more significant differences in operation group compared with control,sham and treatment group（P ＜ 0.01）,that is Cx40 and basilar arteries diameters are reduced,Cx43 and Cx45 are elevated.But the differences in and between Control,sham and treatment group are not obvious （P ＞ 0.05）; Cx37 can＇t examined; Cx40 and basilar arteries diameters are positive correlation（P ＜0.01,r =0.823）,with Cx43 and Cx45 are negative correlation（P ＜0.01,r =-0.840; P ＜0.01,r =-0.839）.Cx43 and Cx45 with basilar arteries diameters are negative（P ＜0.01,r =-0.828;P ＜0.01,r =-0.823）.Cx43 and Cx45 are positive correlation（P ＜ 0.01,r =0.939）.Conclusions Cx43 and Cx45 proteins play an important role which is promotion in the pathomechanism of cerebral vasospasm after experimental subarachnoid hemorrhage,but Cx40 is opposite; There are correlation among Cx40 and Cx43 Cx45,Cx43 and Cx45 are positively correlated,Cx40 and Cx43,Cx45 are negatively correlated,respectively.Gap junction inhibitor 18β-glycyrrhetinic-acid can relieve cerebral vasospasm after experimental subarachnoid hemorrhage in rats in vivo.
出处
《中华神经外科杂志》
CSCD
北大核心
2014年第11期1169-1174,共6页
Chinese Journal of Neurosurgery
基金
新疆生产兵团博士基金资助（2011BB016）
