摘要
目的观察脑缺血再灌注后多药耐药蛋白(Mrp1)在脑内的表达变化,探讨其与缺血再灌注的关系.方法 24只大鼠随机分为假手术组(n=6)和缺血再灌注后1、3、7d组(n=6).应用颈内动脉线栓法建立大鼠大脑中动脉缺血再灌注模型,以抗Mrp1抗体进行免疫组化染色,检测缺血再灌注后脑组织中Mrp1的表达变化.结果 Mrp1在正常脑内的神经元及胶质细胞中均有表达.缺血再灌注后1d、3 d、7d组中,缺血再灌注区的皮质、海马及纹状体内可见Mrp1表达升高,阳性细胞数量增多,在3d组达高峰(P<0.05).结论大鼠脑缺血再灌注后细胞中Mrp1的表达增高,可能是细胞受损时的自我保护反应,但同时也可能与临床耐药性的发生有关.
Objective To explore the expression changes of multidrug resistance protein-1 in brain ischemia/reperfusion in rats.Methods The models of ischemia-reperfusion were established by focal middle cerebral artery occlusion（MCAO） method. 24 adult male SD rats were randomly divided into sham and ischemia/reperfusion group（I/F） including 1day,3days,7days after I/F（n=6）;the expression of Mrp1 was observed by immunohistochemistry.Results Mrp1 was expressed in cells in cerebral cortex, hippocampus and corpus striatum of normal brain and ischemia/ reperfusion brain. The over expression of Mrp1 was found in infarct region after I/R,especially in 3 d group（P〈0.05）.Conclusions The over expression of mrp1 can be found in brain after I/R. It suggests that over expression of mrp1 induced by ischemia/ reperfusion injuring brain tissue may be related with neural self-protection or multidrug resistance.
出处
《昆明医科大学学报》
CAS
2014年第10期4-7,共4页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81060101)
云南省科技厅基金资助项目(2013FZ056)
云南省教育厅基金资助项目(2011Y168)
关键词
脑缺血再灌注
MRP1
大鼠
Brain ischemia/reperfusion
Multidrug resistance protein-1
Rat
