摘要
目的:观察祛痰活血汤对非酒精性脂肪性肝病(nonalcoholic fatty liver disease,NAFLD)模型大鼠肝细胞水通道蛋白9(aquaporin 9,AQP9)表达的影响,探讨祛痰活血汤治疗非酒精性脂肪性肝病的机制。方法:选取普通清洁级雄性SD大鼠60只,随机分5组,每组12只,分别为:空白对照组、模型对照组、祛痰活血汤低、中、高剂量组(6,12,16 g·kg-1),高脂饲料喂养10周建立NAFLD模型,造模成功后,ig给药,每日2次,连续2周。采用HE染色法、酶联免疫吸附试验(Elisa)法及反转录PCR(RT-PCR)技术,观察各组大鼠肝脏病理变化、总胆固醇(TC)、甘油三酯(TG)水平及肝细胞中水通道蛋白9(AQP9)的表达。结果:1基因及蛋白水平,NAFLD模型组大鼠肝细胞中AQP9的表达均较空白对照组上调(P<0.05)。祛痰活血汤3个剂量组大鼠肝细胞AQP9表达均下调,其中12 g·kg-1组AQP9 mRNA表达下调较其他两组明显(P<0.05)。2祛痰活血汤3个剂量组大鼠TC,TG水平均较模型组下降(P<0.05),其中16 g·kg-1组TC,TG水平下降较其他两组明显(P<0.05)。33个剂量祛痰活血汤治疗组大鼠肝脏脂肪变性均较NAFLD模型组减轻,16 g·kg-1组脂肪变性较其他两组减轻明显。结论:祛痰活血汤可调节NAFLD大鼠肝细胞中AQP9的表达,并显著降低NAFLD大鼠TC,TG水平,改善肝脏脂肪变性程度。
Objective ： To observe the effect of Qutan Huoxue decocotion （QTHXD） on the expression of aquaporin 9 （AQP9） in the nonalcoholic fatty liver rats, and discuss the mechanism. Method： Sixty SD rats were randomly divided into 5 groups, 12 each group： the blank control group, model control group, QTHXD low, medium and high dose groups （6, 12, 16 g ·kg^-1） , all rats except balnk congtrol group were fed high fat diet for 10 weeks to establish nonalcoholic fatty liver disease （NAFLD） model. After modelingg, the corresponding drugs were ig giventwice a day, for 2 weeks. HE, ELISA and RT-PCR were used to observe the liver pathology changes, total cholesterol （TC） , triglycerides （TG） and the AQP9 mRNA expression in liver cells. Result： ①AQP9 expression in liver cells of NAFLD model group compared with blank control group was raised., but decreased in three QTHXD dose 12 g ·kg^-1 group was lower than the other two groups. ②TC and TG in three QTHXD dose groups deacreased, 16 g ·kg^-1group was lower than the other two groups. ③Hepatic steatosis in the QTHXD different dose groups was reduced compared with NAFLD model group, and hepatic steatosis in 16 g .kg group reduced more markedly than the other two groups. Conclusion： QTHXD could regulate AQP9 expression of liver cells in NAFLD model rats, reduce TC and TG and improve the degree of hepatic steatosis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第23期160-164,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
泸州市科技局基金项目2012-S-38(14/14)
泸州医学院青年基金(泸医院科发[2012]2号)
关键词
水通道蛋白9
祛痰活血汤
非酒精性脂肪肝
aquaporin 9
Qutan Huoxue decoction
nonalcoholic fatty liver disease
