摘要
目的:通过检测水通道蛋白9(AQP9)及甘油激酶(GK)表达的变化,探讨滋肝阴方一贯煎抗肝癌的机理,并比较一贯煎、化疗及一贯煎化疗联合抗肝癌机理的异同。方法:雄性昆明小鼠40只,随机均分为模型组、化疗组、一贯煎组、一贯化疗联合组。腋下注射H22细胞建立H22荷瘤模型,一贯煎组造模前两周开始用药(剂量为23g·kg-1·d-1),化疗组(环磷酰胺50mg·kg-1·d-1,隔日1次)及一贯化疗联合组造模后用药,造模14d后处死小鼠称重,取瘤组织称重计算抑瘤率,取脾组织称重计算脾指数,免疫组化检测AQP9及GK蛋白的表达。结果:与模型组比较,一贯煎组、化疗组及一贯化疗联合组均具有显著抑瘤作用(P<0.05或P<0.01);一贯煎组能显著提升脾指数用AQP9蛋白的表达,与模型组比较,有统计学意义(P<0.05);一贯化疗联合组AQP9蛋白表达减少,与化疗组及一贯煎组比较,差异有统计学意义(P<0.05);一贯煎可增加GK蛋白的表达,与模型组及化疗组比较,差异均有统计学意义(P<0.05);一贯化疗联合则会减少GK蛋白的表达,与一贯煎组比较,差异有统计学意义(P<0.05)。结论:一贯煎具有显著抑制肝癌细胞增殖功效,其机理可能是通过滋阴上调AQP9及GK蛋白表达,这可能与该方能增强细胞膜对水的通透性而加速细胞凋亡的速度,并可维持血糖保证重要器官的葡萄糖供给有关。但在促进AQP9及GK蛋白表达方面,一贯煎与化疗药无协同作用。
Objective： By detecting AQP9 and GK expression changes,to investigate the mechanism of nourishing liver yin classical prescription of Yiguanjian,thereby to compares the similaritie and differences of Yiguanjian、CTX and the two combined in the treatment of liver cancer. Methods： 40 male Kunming mice were divided into model group,CTX group,Yiguanjian group and the combination group. Animal models of H22 tumor-bearing mice were induced with the axillary injection of H22 cells,Yiguanjian group started medication at molding two weeks before（ 23 g / kg·d）,CTX group（ 50 mg / kg·2d） and the combination group started medication after molding. After 14 d modeling,kill the mice and weigh the tumor tissue and splenic tissue,calculate the tumor inhibition rate and spleen index,testing the expression of AQP9、GK protein on immunohistochemistry assay. Result： The CTX group,Yiguanjian group and the combination group had obvious antitumor effects（ P〈0. 05 or P〈0. 01）; compared with the model group,Yiguanjian group can significantly increase the spleen index and upregulate the expression of AQP9 protein（ P〈0. 05）; the amount of AQP9 protein expression in combination group at least,the differences were statistically significance compared with the chemotherapy group and Yiguanjiangroup（ P〈0. 05）; Yiguanjian could upregulated expression of GK protein（ P〈0. 05）,but when combination with the CTX,GK protein expression was downregulated compared with the Yiguanjian group（ P〈0. 05）. Conclusion： Yiguanjian had significantly effect on inhibit the proliferation of hepatoma carcinoma cells,and its mechanism maybe connected with increasing the expression of AQP9、GK by nourishing yin to enhance the permeability of cell membrane for water and accelerate cell apoptosis rate and maintaining blood sugar glucose supply to ensure vital organs. But in the promotion of AQP9 and GK protein expression,the consistency of the decoction and chemotherapy drugs had no synergistic effect.
出处
《江西中医药大学学报》
2016年第1期71-74,共4页
Journal of Jiangxi University of Chinese Medicine
基金
国家自然科学基金项目(81260523)
江西省研究生创新基金项目(YC2014-S275)
江西中医药大学研究生创新基金项目(JZYC14A02)
关键词
一贯煎
滋肝阴
肝癌
AQP9
GK
Yiguanjian
Nourish Liver Yin
Liver Cancer
AQP9
GK
