摘要
目的 了解杭州地区儿童人偏肺病毒（hMPV）的分子流行病学特点.方法 收集2011年1月至2013年12月杭州市2所哨点医院儿科呼吸道门诊和住院病例2 593例,门诊病例采集咽拭子1 676份,住院病例采集气管吸取物917份.用实时荧光定量PCR方法检测hMPV核酸,阳性样本用RT-PCR方法对hMPV融合蛋白（F）部分基因片段扩增和序列测定.所测基因序列与GenBank代表株核苷酸同源性比较,并构建基因进化树.结果采用描述性统计和样本率的x2检验.结果 杭州地区呼吸道患儿hMPV检出率为6.51％（169/2 593）.门诊检出率为6.62％（111/1 676）,住院检出率为6.32％（58/917）,检出率差异无统计学意义（x2=0.086,P=0.769）.2岁和3岁组检出率最高,分别为14.14％（28/198）、14.01％（22/158）,与其他组比较差异有统计学意义（x2=38.654,P=0.000）.冬春季检出高于夏秋季（x2=67.032,P=0.000）.测序的88株阳性基因序列与GenBank参考株核苷酸序列同源性为81.6％～ 100.0％,基因进化树分析显示分属于2个基因型的4个亚型.2011-2012年B1亚型占优势,2012年底至2013年A2型为流行.＜1岁组A基因型（67.56％,25/37）与≥1岁组（43.13％,22/51）检出率差异有统计学意义（x2=5.143,P=0.023）.结论 杭州地区儿童呼吸道感染hMPV存在4种基因型,＜1岁组A基因型感染较多,2、3岁组hMPV检出率最高.
Objective To understand the molecular epidemiologic features of human metapnenmovirus （hMPV） in children with respiratory tract infection in Hangzhou.Methods 2 593 throat swabs were collected from patients with respiratory tract infections who visited the hospitals with sentinel surveillance programs from January 2011 to December 2013,including 1 676 outpatients and 917 inpatients.Total nucleic acid was extracted from the specimens and the fusion （F） protein gene of hMPV was amplified by quantitative real-time reverse transcription polymerase chain reaction （RT-PCR）,with positive samples picked to compare with the sequence of hMPV in GenBank,after the sequence of amplification products were determined.Other two types of common respiratory virus were tested using RT-PCR.Results The overall positive rate in this study was 6.51％ （169/2 593）,with 6.62％ （111/1 676） in outpatients and 6.32％ （58/917） in inpatients,but no statistically significant difference was found （x2=0.086,P=0.769）.The rates was 7.01％ in males and 5.72％ in females,with no statistically significant difference in different sex （x2=1.676,P=0.195）.The positive rate was 14.14％ （28/198） in the 2-year-olds,14.01％（22/158） in 3-year olds.The rate in 2-year olds was higher than in other groups,with statistically significant differences between the groups （x2=38.654,P=0.000）.Of the 169 positive cases,153 （90.53％） in the younger than 5 years olds.The rates of infection with hMPV in winter and spring were statistically higher than in summer and autumn （x2=67.032,P=0.000）.The rate of co-infection was 19.52％ （33/169）.88 amplified productions were selected for gene sequence analysis,and the F gene homology were 81.6％-100.0％ with reference strains in GenBank.Data showed that all the 4 viral subtypes：A2 （52.27％,46/88）,B1 （37.51％,33/88）,B2 （9.09％,8/88） and A 1 （1.13％,1/88） co-circulated during the study.However,different subtypes appeared predominant in different ye
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2014年第12期1384-1388,共5页
Chinese Journal of Epidemiology
基金
杭州市科技局资助项目（20110833B36,20120533Q24）
关键词
人偏肺病毒
呼吸道感染
流行病学特征
Human metapneumovirus
Respiratory infection
Molecular epidemiology
