摘要
目的 对 2 0 0 4年 4月起在江苏苏北及其邻近地区发生中、小学生爆发急性呼吸道疾病(ARD)流行的集中发热并伴有头痛、头晕、咽痛、扁桃体炎病例进行病因研究。方法 对流行区 2 0位患者的咽拭子标本分离病原体 ,并对阳性分离株进行免疫荧光、聚合酶链反应 (PCR)以及电镜检测。结果 MDCK(35代 )、LLC MK2、VeroE6、MRC 5 (39代 )分离培养均未出现细胞病变 ;军团菌培养亦未发现阳性结果 ;经人喉癌传代细胞 (Hep 2 )分离培养 ,2 0份标本中 13份出现了明显的细胞病变效应 ,电镜下可见细胞胞核内大量呈晶格状排列的典型腺病毒颗粒 ,直接免疫荧光试验及PCR检测均证实为腺病毒感染。结论 2 0 0 4年 4月发生在江苏省苏北地区中、小学急性呼吸道感染疫情的病原体为腺病毒。
Objective Etiology study were conducted on 20 cases with acute respiratory disease (ARD). Methods 20 throat swab samples were collected and the virus isolation was engaged with MDCK, LLC-MK2, Vero, MRC-5 and Hep-2 cells. Bacterial isolation work was conducted at the same time, including legionella and blood plate culture. Results 13 ADV-like cytopathic effect were detected in Hep-2 cultures, and negative staining electron microscopy result typical ADV partials. IFA, PCR also gave out positive result. Conclusions Pathogen of this ARD outbreak in the north of Jiangsu province was adenovirus.
出处
《疾病控制杂志》
2004年第6期486-488,F003,共4页
Chinese Journal of Disease Control and Prevention
