摘要
目的:探讨益气温阳、活血利水方药对充血性心力衰竭(CHF)大鼠模型胰岛素抵抗(IR)及心肌组织病理学改变的影响。方法:通过对Wistar雄性大鼠尾静脉注射盐酸阿霉素制备充血性心力衰竭大鼠模型,造模成功后将Wistar雄性大鼠随机分成模型组、西药组、方药低剂量组、方药中剂量组、方药高剂量组,连续灌胃4周后,取内眦静脉窦静脉血3 m L,测血糖及血清胰岛素,计算IR。最后处死大鼠,取出心脏,切片HE染色,观察心肌组织病理学改变。结果:与模型组比较,益气温阳、活血利水方药能明显改善CHF大鼠IR水平(P<0.05),其中高、中剂量组较为明显;同时益气温阳、活血利水方药能改善CHF大鼠心肌细胞的损伤,高剂量组心肌细胞形态学的改变接近地高辛组。结论:益气温阳、活血利水方药能一定程度逆转大鼠CHF的IR并改善心室重构。
This study was aimed to investigate the effect of Yi-Qi Wen-Yang（YQWY） and Huo-Xue Li-Shui（HXLS） decoction on changes of insulin resistance（IR） model and the myocardial tissues of rats with congestive heart failure（CHF） pathology. CHF rat models were established on Wistar male rats through injection of doxorubicin hydrochloride into rat tail vein. Wistar male rats models were randomly divided into the model group, western medicine group, low dose decoction group, middle dose decoction group, and high dose decoction group. After 4-week gavage, 3 m L vein blood was taken from the angular vein sinus for the determination of blood glucose and serum insulin, and the calculation of IR. Finally, the rats were sacrificed. And then, the heart was removed to make HE slice and observe the pathological change of myocardium. The results showed that compared with the model group, YQWY and HXLS decoction can improve the IR level among CHF rats（P〈0.05）. Among them, the effects of the high dose and middle dose group were obvious. At the same time, this decoction can improve the myocardial cells in CHF rats in myocardial cells of the high dose group. And its morphology change was close to the digoxin group. It was concluded that YQWY and HXLS decoction can reverse IR and improve ventricular remodeling among CHF rats to a certain extent.
出处
《世界科学技术-中医药现代化》
北大核心
2014年第10期2157-2161,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委地区基金项目(81260586):基于"久病及肾"探讨维生素D轴在压力超负荷心肌重塑中的时相差异及康心汤干预机制
负责人:陈云志
贵州省科学技术委员会科技基金(黔科合J字[2012]2064号):基于维生素D及其受体探讨康心汤抗心肌重塑的机制
负责人:陈云志
贵州省中医药管理局中医药
民族医药科学技术专项研究(QZYY2010-32):益气温阳
活血利水方药对充血性心力衰竭大鼠模型IR及心肌组织病理学改变的影响
负责人:王瑶瑶
