摘要
目的：探讨骨形成蛋白－2（BMP－2）对体外培养的人牙髓细胞（DPCs）增殖和分化的影响。方法：体外培养人DPCs，分别与不同浓度的BMP－2（50、100、200 ng／mL）共同培养；分别检测各组细胞的增殖情况、碱性磷酸酶（alkaline phosphatase，ALP）活性、钙化结节形成量以及牙本质涎磷蛋白（DSPP）、牙本质基质蛋白－1（DMP－1）各成牙本质相关基因的表达水平。结果：50～200 ng／mL 的BMP－2对DPCs的增殖均无明显促进作用；但是，能呈剂量依赖性地提高细胞的ALP活性、促进钙化结节的形成、上调DSPP和DMP－1 mRNA的表达水平，各浓度BMP－2组均高于对照组（P ＜0．05）。结论：BMP－2对体外培养的人DPCs增殖无明显影响，但可明显促进DPCs的成牙本质细胞方向分化。
AIM：To investigate the effects of bone morphogenetic protein-2 （BMP-2）on the biologic ac-tivity of human dental pulp cells （DPCs）in vitro.METHODS：Human DPCs were in vitro cultured .The cells of passage 4 were cultured in normal medium （for cell proliferation assay）or osteogenic medium （for osteogenic differen-tiation assay）containing BMP-2 at 50,100,and 200 ng/mL,respectively.while medium without BMP-2 served as the control.The cell proliferation was examined by MTT method.ALP activity,the quantity of calcified nodules and mRNA expression of DSPP and DMP-1 were evaluated and quantified by RT-PCR.RESULTS：BMP-2 did not show effect on the proliferation of DPCs.BMP-2 at all tested concentrations increased ALP activity （P〈0.05）,the calcified nodules（P〈0.05）and mRNA levels of DSPP and DMP-1 in DPCs （P〈0.05）in a dose-and time-dependent manner. CONCLUSION：BMP-2 can promote odontoblast differentiation of DPCs,but has no significant effect on the prolifera-tion of DPCs.
出处
《牙体牙髓牙周病学杂志》
CAS
北大核心
2014年第10期568-571,共4页
Chinese Journal of Conservative Dentistry
关键词
骨形成蛋白
牙髓细胞
增殖
分化
bone morphogenetic protein （BMP）
dental pulp cells
proliferation
differentiation
