摘要
以流苏树种胚为试材,研究不同种类及浓度的植物生长调节剂对其无菌体系建立,带顶、侧芽茎段伸长以及生根的影响。结果表明,种胚生根最适培养基为WPM+0.1 mg·L-1 NAA+3.0 mg·L-1 GA3+2.0 mg·L-1 6-BA,生根率为94.2%;带顶、侧芽茎段最适培养基为WPM+1.0 mg·L-1 GA3+1.0 mg·L-1 6-BA+0.5 mg·L-1 TDZ;生根最适培养基为WPM+0.1 mg·L-16-BA+0.5 mg·L-1 NAA+0.5 mg·L-1 IBA。
Using the embryo of Chionanthus retusus as the material, the effects of different concentrations and combinations of plant growth regulators on asepsis system establishment, stem segment elongation of terminal bud and lateral bud, and rooting were discussed. The results showed that the best embryo rooting medium was WPM＋1.0 mg·L^-1 NAA＋3.0 mg·L^-1 GA3＋2.0 mg·L^-1 6-BA, the rooting rate was 94.2%, the best medium for ter- minal and laterd bud was WPM＋1.0 mg·L^-1 GA3＋1.0 mg·L^-1 6-BA＋0.5 mg·L^-1TDZ, and the best rooting medi- um was WPM＋0.1 mg·L^-16-BA＋0.5 mg·L^-1 NAA＋0.5 mg·L^-1 IBA.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第10期1510-1514,共5页
Plant Physiology Journal
基金
河南省科技攻关计划项目(090080021023)
关键词
流苏树
种胚
无菌体系
Chionanthus retusus
embryo
tissue culture
